Betulinic Acid from Zizyphus Joazeiro Bark Using Focused Microwave-Assisted Extraction and Response Surface Methodology Fernanda Carolina Sousa Fonseca, Letícia Caribé Batista Reis, Jener David Gonçalves dos Santos,
1, 2, 2, 1★★ Corresponding author
- 1Department of Health, State University of Feira de Santana, Feira de Santana, Brazil.
- 2Department of Organic Chemistry, Federal University of Bahia, Salvador, Bahia, Brazil.
CORRESPONDENCE
Alexsandro Branco
Department of Health, State University of Feira de Santana, Feira de Santana, Brazil.
Received: 02-11-2015; Revised: 08-02-2016.
Volume 13, Issue 50 · pp. 226–229 · 2017 · DOI: 10.4103/0973-1296.204565
ABSTRACT
Background: The effect of the extraction time (min) and temperature (ºC) on the yield of betulinic acid (BA) from Zizyphus joazeiro barks using focused microwave-assisted extraction was investigated.Materials and Methods: The ethyl acetate was used as extractor solvent because it was shown to provide a betulinic acid-clean extract. A full two-level statistical factorial design was applied to determine the important effects and interactions of these independent variables upon the yield of BA.Results: The conditions that produced the highest yield of BA were at temperature of 70 ºC and an extraction time of 15 min (3.33 mg per gram of plant).Conclusion: The BA has drawn attention due to its use as a raw material in the synthesis of active compounds against the Human Immunodeficiency Virus (HIV).
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Branco, C. R. C., Ferreira, S. L. D. C., David, J. M., & Branco, A. (2017). Betulinic Acid from Zizyphus Joazeiro Bark Using Focused Microwave-Assisted Extraction and Response Surface Methodology Fernanda Carolina Sousa Fonseca, Letícia Caribé Batista Reis, Jener David Gonçalves dos Santos,. Pharmacognosy Magazine, 13(50), 226–229. https://doi.org/10.4103/0973-1296.204565