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Pharmacognosy MagazineNOW IN
Article

A HPLC-DAD method for the simultaneous determination of five marker components in the traditional herbal medicine Bangpungtongsung-san

2★, 2, 4, 2,3★ Corresponding author

  1. 1Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, South Korea.
  2. 2Research Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, South Korea.
  3. 3TKM Converging Research Division, Korea Institute of Oriental Medicine, Daejeon 305-811, South Korea.

CORRESPONDENCE

Jin Bae Weon

Research Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, South Korea.

Received: 22-07-2010; Revised: 18-09-2010.

Volume 7, Issue 25 · pp. · 2011 · DOI: 10.4103/0973-1296.75903

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ABSTRACT

Background: Bangpungtongsung-san, one of the traditional herbal medicines, was known to be a prescription for obesity.Objective: For the simultaneous determination of five components (paeoniflorin, 6-gingerol, decursin, geniposide, and glycyrrhizin) in Bangpungtongsung-san, a high-performance liquid chromatography with diode-array detector method was established.Materials and Methods: To develop the method, a reverse phase column, DIONEX C18 (5 μm, 120 Å, 4.6 mm × 150 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 230, 240, and 254 nm. Method validation was accomplished by linearity, precision test, and recovery test.Results: All calibration curves of components showed good linearity (R2 > 0.9959). The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 0.17 µg/ml and 0.04 to 0.53 µg/ml, respectively. The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%. In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.Conclusion: This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.

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    Weon, J. B., Yang, H. J., Ma, J. Y., & Ma, C. J. (2011). A HPLC-DAD method for the simultaneous determination of five marker components in the traditional herbal medicine Bangpungtongsung-san. Pharmacognosy Magazine, 7(25). https://doi.org/10.4103/0973-1296.75903