jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Asian Journal of Biological and Life sciencesNOW IN
Article

Antioxidant Potential, Antidiabetic, and Anti-Inflammatory Activities of White Cowpea (Vigna unguiculata L.): An in vitro Study

R. Durga Priyadarshini1, D. Annette Beatrice1★, C. Sivaraj2★ Corresponding author

  1. 1Department of Home Science, Women’s Christian College (Affiliated to the University of Madras), Chennai, Tamil Nadu, INDIA.
  2. 2ARMATS Biotek Training and Original Institute, Chennai, Tamil Nadu, INDIA.

CORRESPONDENCE

D. Annette Beatrice

Department of Home Science, Women’s Christian College (Affiliated to the University of Madras), Chennai, Tamil Nadu, INDIA.

annettebeatrice@yahoo.com

Received: 14-09-2023; Revised: 15-10-2023; Accepted: 16-11-2023.

Volume 12, Issue 3 · pp. 564–573 · PUBLISHED · DOI: 10.5530/ajbls.2023.12.74

View on AJBLS original site ↗

ABSTRACT

Aim/Background:Legumes are nutrient-dense with bioactive compounds offering protective effects against non-communicable diseases. The study aimed to screen phytochemicals, determine the total phenol and flavonoid contents and assess thein vitroantioxidant potential, and antidiabetic and anti-inflammatory activity of white cowpeas.Materials and Methods:Aqueous and ethanolic extracts of white cowpea were prepared. Total phenol content was estimated using the Folin- Ciocalteu colorimetric method and total flavonoid content was estimated using the aluminium chloride method. The antioxidant potential was assessed using DPPH assay, superoxide radical scavenging assay, phosphomolybdenum reduction assay and FRAP assay. The antidiabetic activity was evaluated using an alpha-amylase inhibition assay and the anti-inflammatory activity was evaluated using a human red blood cells membrane stabilization assay.Results:The total phenolic and total flavonoid content of white cowpea was two folds higher in the ethanol extract (343.67±1.19 μg/mg GAE and 60.87±0.33 μg/mg QE, respectively) than aqueous extract. Antioxidant analysis revealed that the ethanol extract exerted superior activity with an IC50 of 47.21±0.09 μg/mL against DPPH, 110.6±0.2 μg/mL against superoxide anions, 49.62±0.26 μg/ mL against ferric while the aqueous extract had superior activity against phosphomolybdenum with an IC50 of 30.05±0.02 μg/mL. The anti-diabetic and anti-inflammatory analysis revealed superior activity of ethanol extract (30.88±0.51 μg/mL; 97.99±3.34 μg/mL, respectively) on par with the standard (acarbose and aspirin, respectively). The principal bioactive compounds present in white cowpea are ethyl α-d-glucopyranoside (43.24%), melezitose (18.25%) and 3-O-Methyl-d-glucose (11.19%).Conclusion:White cowpeas are rich in bioactive compounds with excellent antioxidant potential, anti-diabetic and anti-inflammatory activity.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Priyadarshini, R. D., Beatrice, D. A., & Sivaraj, C. (). Antioxidant Potential, Antidiabetic, and Anti-Inflammatory Activities of White Cowpea (Vigna unguiculata L.): An in vitro Study. Asian Journal of Biological and Life sciences, 12(3), 564–573. https://doi.org/10.5530/ajbls.2023.12.74