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Asian Journal of Biological and Life sciencesNOW IN
Article

Effect of Alpha-Phellandrene on Glucose Uptake and Adipogenesis in Insulin Resistant 3T3-L1 Adipocytes: an in vitro and in silico Approach

Souprayen Seethalakshmi1, Ravishankar Sarumathi1, Chandrasekaran Sankaranarayanan1★★ Corresponding author

  1. 1Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, INDIA.

CORRESPONDENCE

Chandrasekaran Sankaranarayanan

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, INDIA.

sankarhari05@gmail.com

Received: 11-09-2023; Revised: 17-10-2023; Accepted: 15-11-2023.

Volume 12, Issue 3 · pp. 603–610 · PUBLISHED · DOI: 10.5530/ajbls.2023.12.79

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ABSTRACT

Aim:The present study was designated to investigate the effect of alpha-phellandrene, a monoterpene presents in essential oils of mint, turmeric, black pepper, and cinnamon on adipocyte function under insulin resistant condition.Materials and Methods:Mature 3T3-L1 adipocytes were exposed to high glucose (25 mM) and 0.6 nm/L of insulin for 24 hr to convert to IR-3T3-L1 adipocytes. The adipocytes were grouped into group1: normal control, group 2: diabetic control, group 3: diabetic group treated with alpha- phellandrene (65 μM) and group 4: diabetic group treated with rosiglitazone (0.1 μM). Glucose uptake assay, triglyceride accumulation and the activity of glycerol-3-phosphate dehydrogenase were measured. The binding affinity of alphaphellandrene with PPARγ and SREBP-1c were analysed by docking studies by using Auto Dock vina (V. 4.0) in Pyrx software.Results:Glucose uptake, triglyceride accumulation and activity of glycerol- 3-phopshate dehydrogenase were found to be decreased in diabetic control when control to normal control group. Alpha-phellandrene at dose of 65 μM increased glucose uptake, enhanced glycerol-3-phosphate activity and triglyceride accumulation in adipocytes which were found to be comparable with the standard drug rosiglitazone. A high binding affinity of alpha-phellandrene with key transcription factors, PPARγ and SREBP-1c which are associated with adipogenesis and steroidogenesis were observed in molecular docking studies.Conclusion:These results suggest that alpha-phellandrene positively regulates adipocyte function and ameliorate dyslipidaemia and hyperglycaemia in type II diabetes mellitus.

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    Seethalakshmi, S., Sarumathi, R., & Sankaranarayanan, C. (). Effect of Alpha-Phellandrene on Glucose Uptake and Adipogenesis in Insulin Resistant 3T3-L1 Adipocytes: an in vitro and in silico Approach. Asian Journal of Biological and Life sciences, 12(3), 603–610. https://doi.org/10.5530/ajbls.2023.12.79