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Asian Journal of Biological and Life sciencesNOW IN
Article

In vivo Antibacterial Activity of Biosynthesized Selenium Nanoparticles using a Zebrafish Model

Christa Judiel Ramos Amor1★, Ronee Anne Angala Abaya1, Charo Mikaella Atencio Alcera1, Bien Jaric Valdez Antonio1, Tatum Ranile Bautista1, Princes Mae Oborsa Baximen1, Gabrielle Frances Cruz Berido1, Joshua Carl Lim Boe1, Pamela Rose Flores Bremner1, RMT R1, MSMT T1★ Corresponding author

  1. 1Department of Medical Technology, Far Eastern Unaiversity, Nicanor Reyes Sr, Street, Sampaloc, Manila, Metro Manila, PHILIPPINES.

CORRESPONDENCE

Christa Judiel Ramos Amor

Department of Medical Technology, Far Eastern Unaiversity, Nicanor Reyes Sr, Street, Sampaloc, Manila, Metro Manila, PHILIPPINES.

christajudielamor@gmail.com

Received: 14-06-2023; Revised: 07-07-2024; Accepted: 13-08-2024.

Volume 13, Issue 2 · pp. 338–346 · PUBLISHED · DOI: 10.5530/ajbls.2024.13.43

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ABSTRACT

Background:Staphylococcus aureusis a leading cause of both healthcare-associated and community-acquired infections, contributing to an extensive array of illnesses, ranging from skin infections to life-threatening conditions like pneumonia and bloodstream infections. Despite extensive efforts, its resistance to multiple antibiotics poses a significant public health threat. This paper employed an experimental design to explore the potential of nanotechnology, specifically selenium nanoparticles, in combating in vivoantibacterial resistance of Staphylococcus aureus(S. aureus) infections using a zebrafish model. Materials and Methods:Selenium Nanoparticles (SeNPs) were synthesized from orange peel waste extract and sodium selenite. 93 zebrafish were infected with S. aureusvia intramuscular injection and then exposed to various concentrations of SeNPs via medicated bath using Ciprofloxacin as positive control. Following the IACUC guidelines for euthanasia, fish muscle was cultured using MSA and then antimicrobial activity was noted in CFUs, or colony-forming units. Results:Results indicated significant antibacterial activity at all selenium nanoparticle concentrations, with 20 μg/mL showing the most pronounced effect, notably reducing colony counts compared to the negative control. Statistical analysis demonstrated differences between selenium nanoparticle concentrations, particularly with 15 μg/mL and 20 μg/mL having shown similar effectiveness. Conclusion:Overall, selenium nanoparticles exhibited a high level of antimicrobial activity against S. aureus, with an effectiveness rate of 95.9%. Antibacterial Selenium nanoparticles Zebrafish.

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    Amor, C. J. R., Abaya, R. A. A., Alcera, C. M. A., Antonio, B. J. V., Bautista, T. R., Baximen, P. M. O., Berido, G. F. C., Boe, J. C. L., Bremner, P. R. F., R, R., & T, M. (). In vivo Antibacterial Activity of Biosynthesized Selenium Nanoparticles using a Zebrafish Model. Asian Journal of Biological and Life sciences, 13(2), 338–346. https://doi.org/10.5530/ajbls.2024.13.43