Phytochemical Potential of Heliotropium marifolium (J. kocnig ex Retz.)- An in vitro Antioxidant and Antidiabetic Analysis
Srinivasan Nithya1★, Edward Gnanaraj Wesely1, Helan Soundra Rani Michael2, Musthapha Shabana Begum3★ Corresponding author
- 1Department of Botany, Arignar Anna Government Arts College, Namakkal, Tamil Nadu, INDIA.
- 2Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, INDIA.
- 3Sri Shanmugha Institute of Medical Science and Research, University in Puthur, Tamil Nadu, INDIA.
CORRESPONDENCE
Srinivasan Nithya
Department of Botany, Arignar Anna Government Arts College, Namakkal, Tamil Nadu, INDIA.
Received: 18-08-2025; Revised: 28-10-2025; Accepted: 02-12-2025.
Volume 14, Issue 3 · pp. 666–671 · PUBLISHED · DOI: 10.5530/ajbls.20250060
ABSTRACT
This study aimed to assess the antioxidant, anti-inflammatory, and α-amylase inhibitory activities of H. marifolium leaf and stem extracts using in vitro assays to validate its pharmacological potential. Antioxidant assays including DPPH, ABTS, hydrogen peroxide, superoxide, hydroxyl, and nitric oxide radical scavenging tests were conducted at different extract concentrations (50–300 µg/mL). Anti-inflammatory activity was assessed via protein denaturation inhibition, and α-amylase inhibition was determined using starch–iodine complex assays. Ascorbic acid and acarbose were used as standards. Both extracts exhibited concentration-dependent antioxidant activity, with the leaf extract showing superior scavenging efficiency across all radicals (DPPH, 88.32 ± 1.66%; H₂O₂, 91.29 ± 1.70%) and lower IC₅₀ values (130.52–181.27 µg/mL) compared with the stem extract. The anti-inflammatory assay revealed significant inhibition (79.90 ± 1.59%) for the leaf extract at 300 µg/mL, with an IC₅₀ of 178.30 µg/mL versus 202.18 µg/mL for the stem. In the α-amylase inhibition assay, the leaf extract (IC₅₀ = 187.30 µg/mL) again showed higher potency than the stem (IC₅₀ = 209.26 µg/mL), though both were lower than acarbose (IC₅₀ = 122.37 µg/mL). The findings demonstrate that Heliotropium marifolium leaf extract possesses strong antioxidant, anti-inflammatory, and α-amylase inhibitory properties, supporting its potential as a natural source of bioactive compounds for managing oxidative stress, inflammation, and hyperglycemia.
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Nithya, S., Wesely, E. G., Michael, H. S. R., & Begum, M. S. (). Phytochemical Potential of Heliotropium marifolium (J. kocnig ex Retz.)- An in vitro Antioxidant and Antidiabetic Analysis. Asian Journal of Biological and Life sciences, 14(3), 666–671. https://doi.org/10.5530/ajbls.20250060