Simultaneous Determination of Linezolid and Levamisole Hydrochloride in a Fixed Dose Combination
Lalit Kumar1, Yugvijay Singh Yadav1, Mahalaxmi Rathnanand1★★ Corresponding author
- 1Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Madhav Nagar 576 104, Manipal, Udupi, Karnataka, INDIA.
CORRESPONDENCE
Mahalaxmi Rathnanand
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Madhav Nagar 576 104, Manipal, Udupi, Karnataka, INDIA.
Received: 20-03-2017; Revised: 02-05-2017; Accepted: 13-07-2017.
Volume 51, Issue 4 · pp. 613–619 · PUBLISHED 2017 · DOI: 10.5530/ijper.51.4.91
ABSTRACT
Background: The combination of antibiotics with an immune-stimulant can be used for the prevention of antimicrobial resistance. Multilayer tablets of linezolid and levamisole hydrochloride can be a solution of this problem. Objective: Aim of the present study was to High Performance Liquid Chromatography method development and validation as per the International Council for Harmonisation guidelines for analysis of both the drugs simultaneously in a single unit dosage form. Methods: Ammonium acetate and acetonitrile in the ratio 65:35 (%v/v) was used as mobile phase. Ultra violet detection was carried out at 236 nm and flow rate was kept as 0.9 mL/min. Results: Linezolid and levamisole hydrochloride were found at 4.61 min and 6.45 min retention times, respectively. Conclusion: The study confirmed that the present method can be used for routine simultaneous analysis of linezolid and levamisole hydrochloride.
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Kumar, L., Yadav, Y. S., & Rathnanand, M. (2017). Simultaneous Determination of Linezolid and Levamisole Hydrochloride in a Fixed Dose Combination. Indian Journal of Pharmaceutical Education and Research, 51(4), 613–619. https://doi.org/10.5530/ijper.51.4.91