jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Novel RP-HPLC Method Development and Validation of Meloxicam Suppository

Sufiyan Ahmad1★, Sharma Deepika1, Patil Amol1, Warude Kapil1, Md. Rageeb Md.Usman2★ Corresponding author

  1. 1Department of Quality Assurance, Gangamai College of Pharmacy, Nagaon, Dist-Dhule (Maharashtra), INDIA.
  2. 2Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Maharashtra, INDIA.

CORRESPONDENCE

Sufiyan Ahmad

Department of Quality Assurance, Gangamai College of Pharmacy, Nagaon, Dist-Dhule (Maharashtra), INDIA.

sufimpharm@rediffmail.com

Received: 09-02-2017; Revised: 14-03-2017; Accepted: 13-07-2017.

Volume 51, Issue 4 · pp. 644–649 · PUBLISHED 2017 · DOI: 10.5530/ijper.51.4.95

View on IJPER original site ↗

ABSTRACT

Objective: A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Meloxicam drug (MLX) in pharmaceutical mixture. Methods: Effective chromatographic separation achieved using a phenomenex luna C18 (4.6 mm, 250 mm, 5 μm) column with isocratic elution by the mobile phase composed of 0.02 M Potassium dihydrogen orthophosphate, pH adjusted to 4 with orthophosphoric acid (filtered): acetonitrile (50:50) respectively. The flow rate is 1.0 ml/min on detecting wavelength 220 nm. Results: The proposed HPLC method was statistically validated with respect to linearity, ranges, precision, accuracy, selectivity, LOD, LOQ and robustness. The retention time (RT) of Meloxicam was found to be 6.0 min. respectively. All parameters were found to be within the acceptance limit. The calibration curve was linear in ranges of 3-6, 6-9, and 15-18 mg/ ml for Meloxicam. The R2 of Meloxicam was found to be 0.996 respectively. Conclusion: A novel simple, simple, sensitive, precise, rapid, accurate and economical and reliable RP-HPLC method was developed and validated for the Meloxicam suppository.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Ahmad, S., Deepika, S., Amol, P., Kapil, W., & Md.Usman, M. R. (2017). Novel RP-HPLC Method Development and Validation of Meloxicam Suppository. Indian Journal of Pharmaceutical Education and Research, 51(4), 644–649. https://doi.org/10.5530/ijper.51.4.95