Prolonged in vivo Stinging Nettle Treatment Impacts on Functional Capacity of Leukocytes in Immunologically Mature Chickens
Șandru Carmen Dana1, Niculae Mihaela1, Pall Emoke1, Brudaşcă Florinel1, Vasiu Aurel1, Silvana Popescu1, Cerbu Constantin1, Giupana Radu1, Vasiu Constantin1, Valentin Tudor1, Spînu Marina1★★ Corresponding author
- 1Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
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Spînu Marina
Department of Clinical Sciences - Infectious diseases, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur no.3-5, Cluj-Napoca, ROMANIA.
Received: 30-08-2017; Revised: 17-11-2017; Accepted: 23-11-2017.
Volume 52, Issue 4S · pp. s91–s95 · PUBLISHED 2018 · DOI: 10.5530/ijper.52.4s.81
ABSTRACT
Objective:The experiment aimed at establishing the effects of an alcoholic Urtica dioica extract on the in vitro blastogenic response in antigen stimulated, immunologically mature chickens. Materials and Methods: Three equal groups (n=17) of 47 days old, Rock x Cornish chickens were subjected to oral administration of: a) 0.5 ml/chicken/ day of an alcoholic stinging nettle extract (I), b) 0.5 ml/chicken/day alcohol (II, solvent control), and c) 0.5 ml chicken/day water (III-environment control) for seven days. All birds were injected with 0.5 ml/bird of a 5% SRBC suspension (days 0 and 7). Leukocyte numbers (Burker Turk method) and their blastogenic capacity (blast transformation test) were monitored on days 0, 7 and 14. The specific response was evaluated against a SRBC lysate. Student’s t-test was used to evaluate the statistical significance of the differences. Results and Discussion: The leukocyte numbers increased to from 18,083.33 ±4,879.81/mm3 to 42,833.33±7,547.99/mm3 by day 14. In vitro responses to C. officinalis and E. angustifolia decreased while SRBC response increased in the nettle treated group. Conclusion: The results did not validate the implemented protocol for the alcoholic stinging nettle treatment. Other administration routes, schemes or dosages should be tested to improve the functional capacity of the leukocytes.
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Dana, A. C., Mihaela, N., Emoke, P., Florinel, B., Aurel, V., Popescu, S., Constantin, C., Radu, G., Constantin, V., Tudor, V., & Marina, S. (2018). Prolonged in vivo Stinging Nettle Treatment Impacts on Functional Capacity of Leukocytes in Immunologically Mature Chickens. Indian Journal of Pharmaceutical Education and Research, 52(4S), s91–s95. https://doi.org/10.5530/ijper.52.4s.81