Simultaneous Determination of Hydrophilic and Lipophilic Drugs in Anti-Cancer Liposomes: Absorptivity Method

ABSTRACT

Background: Dual loaded liposomes have always been a hot topic of investigation because of their therapeutic advantages over single drug regimens. But simultaneous estimation of both the drugs during in vitro and in vivo analysis is most of the times a major obstacle for the researchers. This problem is even bigger when the two drugs are of opposite nature (hydrophilic and lipophilic). Methods: Here, we formulated anti-cancer liposomes loaded with Dacarbazine (hydrophilic) and Eugenol (lipophilic). Since both the drugs are of different nature, selecting a solvent system that dissolves both the drugs, as well as is a suitable media for determining in vitro release, was a challenge. After resolving this issue, we developed a simple and robust UV method based on the principle of solving simultaneous equations generated after Absorptivity measurements of the two drugs. Results: UV spectrophotometer is preferred over other analytical techniques because of its availability, broad area of applications, easy and fast handling, robust nature and simplicity. Thus, using the developed method for simultaneous determination, loading of the two drugs and their simultaneous release from the liposomes was determined. Conclusion: We could perform the in-vitro characterization of formulated anti-cancer liposomes using the developed UV Absorptivity method, which justifies the practical applicability of the developed method and proved that the method is specific and reproducible.

KEYWORDS

• UV absorptivity method
• Simultaneous determination
• Dual loaded liposomes
• Suitable solvent system
• Method Validation

Showing references and in-text citations exactly as published.

• DOI10.5530/ijper.53.2s.63Copy

RELATED ARTICLES

Matched on section and article type within this issue of IJPER.

• Article · VOLUME 53, ISSUE 2SNovel Preparation and Effective Delivery of Mucoadeshive Nanoparticles Containing Anti-diabetic DrugSree Harsha Nagaraja, Bandar E Al-Dhubiab, Rakesh Kumar Tekade, Katharigatta Narayanaswamy Venugopala, Ravindra Vasant Ghorpade, Girish Meravanige, Ahmed Alqadheeb · pp. s43–s49Original ArticleSame type: Article
• Article · VOLUME 53, ISSUE 2SFormulation and Characterization of Controlled Release Flurbiprofen Microsponges Loaded in GelsAnkita Vernekar, Rajashree Gude, Nidhi Ghadi, Steffi Parab, Akshata Shirodker · pp. s50–s57Original ArticleSame type: Article
• Article · VOLUME 53, ISSUE 2SEffect of Sustained Release Polymers on Drug Release Profile of Aceclofenac TabletsPhysicochemical Properties, Analysis of Kinetics and Fit FactorUzair Nagra, Sherjeel Adnan, Sana Shafqat, Maryam Shabbir, Sajid Ali, Anum Zafar, Syed Saeed ul Hassan, Javed Iqbal · pp. s58–s65Original ArticleSame type: Article
• Article · VOLUME 53, ISSUE 2SSustained Release Bioadhesive Ocular Inserts of Olopatadine Hydrochloride: Formulation and CharacterizationVidya Sabale, Omprakash Nanalaji Kumawat, Prafulla Madhukarrao Sabale · pp. s66–s73Original ArticleSame type: Article