Antiproliferative Effect of Crude Venom from Conus virgo on Human Lung Cancer Cell Line and Toxicity Assessment on Adult Zebra Fish (Danio rerio)
Dhana Sekaran Ganesan1, Mohamed Asik Raj Mohamed1, Pema Lhanzin1, Kathirvel Neelan1, Lakshmanan Vadakkuvaselvi1, Sellamuthu Subramani1, Arulvasu Chinnasamy1★★ Corresponding author
- 1Unit of Animal Tissue Culture and Aquaculture, Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu, INDIA.
CORRESPONDENCE
Arulvasu Chinnasamy
Unit of Animal Tissue Culture and Aquaculture, Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu, INDIA.
Received: 28-02-2019; Revised: 24-05-2019; Accepted: 16-10-2019.
Volume 54, Issue 1 · pp. 85–94 · PUBLISHED 2020 · DOI: 10.5530/ijper.54.1.10
ABSTRACT
Background: For the past two decades, few conotoxins entered in clinical trial and some are available for pain relieving as well as for treatment of neurological disorders. Aim: The present investigation was made to elucidate the antiproliferative effect of crude venom from Conus virgo against human lung adenocarcinoma cells. Acute toxicity was determined on adult Zebra fish. Methods: Cytotoxicity was assessed by MTT assay where the DNA damage was carried out by DNA fragmentation method and the in vivo toxicity was assessed by OECD guidelines. Results: The cell viability was found to decrease and the cell lost their morphological integrity with increasing concentration (10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 μg/ml) with an IC50 concentration of 74.69 μg/ml. The nuclear integrity and DNA fragmentation assay revealed the induction of DNA damage which was observed at 5 h. Further acute toxicity study was made, the LD50 was found at a concentration of 96.393μg/ml. The crude venom affects the locomotory activity of adult Zebra fish and induces the paralytic activity at 50, 75, 100 and 125 μg/ml concentrations. Histology of brain tissue indicated slight variations in the pallium. Conclusion: From the investigation, it was inferred that C. virgo venom could be a potential candidate for cancer chemotherapy.
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Ganesan, D. S., Mohamed, M. A. R., Lhanzin, P., Neelan, K., Vadakkuvaselvi, L., Subramani, S., & Chinnasamy, A. (2020). Antiproliferative Effect of Crude Venom from Conus virgo on Human Lung Cancer Cell Line and Toxicity Assessment on Adult Zebra Fish (Danio rerio). Indian Journal of Pharmaceutical Education and Research, 54(1), 85–94. https://doi.org/10.5530/ijper.54.1.10