New Bioactive Molecules Isolated for the First Time from Hyoscyamus albus L. and their Mechanisms Underlying the Anticancer Effects
Massinissa Yahia1,2★, Afaf Benhouda2, Mouloud Yahia2, Hamada Haba2, Francisco Campos3, Souhila Benbia2, Antonio Rangel3★ Corresponding author
- 1Department of Biology of Living Organisms, University of BATNA-2-, ALGERIA.
- 2Departments of Pharmacy, University of Naples Federico II, 80131 Naples, ITALY.
- 3Catholic University of Spain, Pharmacy Department Madrid, Murcia, SPAIN.
CORRESPONDENCE
Massinissa Yahia
Department of Biology of Living Organisms, University of BATNA-2-, ALGERIA.
Received: 26-11-2019; Revised: 26-02-2020; Accepted: 15-04-2020.
Volume 54, Issue 2S · pp. s309–s315 · PUBLISHED 2020 · DOI: 10.5530/ijper.54.2s.88
ABSTRACT
Background: Hyoscyamus albus L is a small genus from Solanaceae family known by its use in old traditional medicine in the east of Algeria. Aim: This study aimed to characterize new bioactive molecules from H. albus, evaluate their anticancer activity in several cancer cells and investigate their possible molecular mechanism. Materials and Methods: New compounds (Peak h of fraction F), (Peak 3 of Fraction F), (Peak 1 of fraction C) were isolated from H.albus L. byusinghigh-performance chromatography (HPLC), mass spectrometry (MS) and proton NMR (NMR H1). All isolated compounds were subjected to cytotoxicity and antiproliferative assays against a panel of the four cell lines:DU-145, U-2 OS, U-87 MG and LN-229 cell lines and was determined using MTT assay, Annexin V and propodium iodide was used to evaluate apoptosis. Results: The phytochemical study of H. albus Fractions led to the isolation of quercetin-3-O-β-dglucopyranosyl-( 1 → 6)-β-d-glucopyranosid, N-trans-feruloyltyramine, Hydrocaffeoyl-N8- caffeoylspermidine.The biological results indicated that all cell lines were consistently sensitive to P1 FC in a dose-dependent manner. This difference in cytotoxic sensitivity was more pronounced in osteosarcoma cell line, U-2 OS when compared to prostate cancer and U-87 MG. Cell viability data also demonstrated that only U-87 MG cells were responsive to treatment with Ph FF. compounds P1 FC and Ph FF have induced necrosis and apoptosis in large part of LN-229 cells. Conclusion: The overall results of the present study provided evidence that isolated compounds are potential therapeutic entities against cancer.
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Yahia, M., Benhouda, A., Yahia, M., Haba, H., Campos, F., Benbia, S., & Rangel, A. (2020). New Bioactive Molecules Isolated for the First Time from Hyoscyamus albus L. and their Mechanisms Underlying the Anticancer Effects. Indian Journal of Pharmaceutical Education and Research, 54(2S), s309–s315. https://doi.org/10.5530/ijper.54.2s.88