A Novel Validated Stability Indicating Analytical Method for Simultaneous Quantification of Metformin Hydrochloride and Empagliflozin in Bulk and Marketed Formulation by HPTLC using Box-Wilson Experimental Design Approach

ABSTRACT

Background: A novel stability indicating analytical method was developed and validated by High Performance Thin Layer Chromatography (HPTLC) using Design of experiment approach. The proposed method is useful for quantification of Metformin hydrochloride and Empagliflozin in bulk and its dosage forms simultaneously. Design of experiment approach was applied for optimization of chromatographic conditions. Materials and Methods: For optimization process independent variables were used as Isopropyl alcohol proportion in mobile phase, saturation time of chamber and distance travelled by mobile phase. Experiments were carried out on silica gel pre-coated plate using mobile phase as 2 % Ammonium acetate: Isopropyl alcohol: Triethylamine (4:6:0.1 v/v/v). Direct evaluation of chromatograms were done by TLC scanner with reflectance/absorbance mode set at 242 nm. Method was validated as per ICH Q2 (R1) requirements. Results: Correlation coefficients for calibration curves were found to be 0.985 and 0.988, the calibration curve is in concentration range of 5000-30000 ng band-1 and 125–750 ng band-1 for Metformin hydrochloride and Empagliflozin respectively. The method showed % recovery between 99.05 to 102.54 % for Metformin hydrochloride and 99.20 to 101.50 % for Empagliflozin. The method has a prospective to determine Metformin hydrochloride and Empagliflozin simultaneously. The Metformin hydrochloride and Empagliflozin were subjected to forced degradation studies like hydrolysis, oxidation, thermolysis and photodegradation. Conclusion: Proposed method has capacity to separate the Metformin hydrochloride and Empagliflozin in its degradation products. Hence one can apply this method effectively for routine analysis and during stability study as per regulatory requirements.

KEYWORDS

• Method development
• Validation
• HPTLC
• Stability studies
• DoE

Showing references and in-text citations exactly as published.

• DOI10.5530/ijper.54.3s.164Copy

RELATED ARTICLES

Matched on section and article type within this issue of IJPER.

• Article · VOLUME 54, ISSUE 3SRational Design and Characterization of Transdermal Patch of Irbesartan for HypertensionRoshan Kumar Dubey, Hitesh Kumar Dewangan · pp. s464–s472Original ArticleSame type: Article
• Article · VOLUME 54, ISSUE 3SSolid Dosage Forms: A Detailed Research on Non-conforming Product QualityKatta Hemanth G, Ravindra Shenoy U, Girish Thunga, Sudeep Kumar Agrawal, Mahendra Joshi, Muddukrishna Badamane Sathyanarayana, Vamshi Krishna Tippavajhala, Abhinaya N, Girish Pai Kulyadi · pp. s473–s484Original ArticleSame type: Article
• Article · VOLUME 54, ISSUE 3SEffect of Plasticizer on Delivery of Valsartan from Tamarind Gel Based Transdermal Film FormulationBraja Bihari Panda, Ayan Chatterjee, Rudra Narayan Sahoo, Subrata Mallick · pp. s485–s491Original ArticleSame type: Article
• Article · VOLUME 54, ISSUE 3SEffective Single Drug Treatment of Lymphatic Filariasis through Enhanced Transdermal Delivery of Ivermectin Liposomes using Solid and Dissolving MicroneedlesJyothirmayee Devineni, Ch.Durga Pravallika, Boothapati Sudha Rani, Buchi Naidu Nalluri · pp. s492–s504Original ArticleSame type: Article