Anti-metastatic and Anticancer Potentials of Synthesized Chalcones in B16-F10 Melanoma Cells Induced Metastatic Lung Cancer in C57BL/6 Mice
Nitesh Kumar1,2, Nunsavath Kishan1, Subhankar Biswas1, Karthik Gourishetti1, Mehnaz Kamal3, Mallikarjuna Rao Chamallamudi1★★ Corresponding author
- 1Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, INDIA.
- 2Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Hajipur, Bihar, INDIA.
- 3Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj -11942, KINGDOM OF SAUDI ARABIA.
CORRESPONDENCE
Mallikarjuna Rao Chamallamudi
Principal and Professor of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Karnataka, INDIA.
Received: 12-02-2021; Revised: 10-04-2021; Accepted: 08-08-2021.
Volume 55, Issue 3S · pp. S742–S750 · PUBLISHED 2021 · DOI: 10.5530/ijper.55.3s.181
ABSTRACT
Aim/Background: Metastasis is the main reason of death in cancer with a key challenge to target it precisely by the available treatments. The current study was designed to assess the anti-metastatic potentials of the synthesized chalcones, Ch-1 and Ch-2. Materials and Methods: In vitro study included cytotoxicity, scratch wound assay and cell cycle analysis of Ch-1 and Ch-2. In vivo study was performed in C57BL/6 mice in a B16-F10 induced metastatic lung cancer model. Results: Ch-1 was found to be a potent cytotoxic agent on cell lines namely, MCF-7 (human breast cancer cells), B16-F10 (melanoma cells), Hela (cervical cancer cells) and HEK (human embryonic kidney cells) with IC50 < 100 µM. Ch-1 deterred the G2/M phase of the MCF-7 cell cycle, S Phase of the HeLa cell cycle and G0-G1 phase of the B16-F10 cell cycle. Scratch wound assay in MCF-7 cells with Ch-1 and Ch-2 showed a significant (p<0.05) prevention in their multiplication when compared to untreated cells at both 24 h and 48 h intervals. In vivo study showed that, oral Ch-1 administration at 100 mg/kg b.w. reduced metastatic lung nodules to 77.32%. Standard drug Quercetin at 100 mg/kg b.w. inhibited the lung tumor nodule formation to 56.5%. However, except for WBC count, Ch-1 (100 mg/kg) also displayed significant improvement in hematological profile. No significant difference was found in any of the groups of animals in mean body weight and major organ weight. Conclusion: Study supports the possible use of Ch-1 as a potential anticancer agent in metastatic lung cancer.
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Kumar, N., Kishan, N., Biswas, S., Gourishetti, K., Kamal, M., & Chamallamudi, M. R. (2021). Anti-metastatic and Anticancer Potentials of Synthesized Chalcones in B16-F10 Melanoma Cells Induced Metastatic Lung Cancer in C57BL/6 Mice. Indian Journal of Pharmaceutical Education and Research, 55(3S), S742–S750. https://doi.org/10.5530/ijper.55.3s.181