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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Development of Fast, Precise and Selective RP-HPLC Methods for Identification of Possible Degradation Products of Ivermectin in Isolated Rat Hepatocytes and in Different pH Media

Alexandrina Dineva1★, Borislav Angelov2, Magdalena Kondeva-Burdina2, Maya Georgieva1, Alexander Zlatkov1★ Corresponding author

  1. 1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University–Sofia, Sofia, BULGARIA.
  2. 2Department of Pharmacology, Toxicology and Pharmacotherapy, Faculty of Pharmacy, Medical University–Sofia, Sofia, BULGARIA.

CORRESPONDENCE

Alexandrina Dineva

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University–Sofia, Sofia, BULGARIA.

a.dineva@pharmfac.mu-sofia.bg

Received: 19-07-2021; Revised: 23-12-2021; Accepted: 17-01-2022.

Volume 56, Issue 2 · pp. 546–552 · PUBLISHED 2022 · DOI: 10.5530/ijper.56.2.77

View on IJPER original site ↗

ABSTRACT

Aim: A new stability indicating RP-HPLC based methods were developed to identify the possible degradation products of Ivermectin in isolated rat hepatocytes and in different pH media using a C18 RP column. Complete validation, including linearity, accuracy, recovery, precision, robustness, stability, and peak purity, was performed. Materials and Methods: HPLC system of UltiMateDionex 3000 DAD with LiChrosorb C18 Column were used in this study. During the investigation, for the removal of interfering fraction of isolated rat hepatocytes a purification procedure, consisting of protein precipitation followed by double filtration was developed. Results: Method I was applied effectively and the results indicated two major hepatic metabolites of Ivermectin. Method II was developed to monitor the chemical stability in close to physiological conditions. The appearance of a new peak and the proportional decrease in the sample concentration indicate a liability in buffer with pH12. Conclusion: The obtained results demonstrated that the degradation products of Ivermectin in hepatic cells and in alkali media are different.

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    Dineva, A., Angelov, B., Kondeva-Burdina, M., Georgieva, M., & Zlatkov, A. (2022). Development of Fast, Precise and Selective RP-HPLC Methods for Identification of Possible Degradation Products of Ivermectin in Isolated Rat Hepatocytes and in Different pH Media. Indian Journal of Pharmaceutical Education and Research, 56(2), 546–552. https://doi.org/10.5530/ijper.56.2.77