Binary Solvent-assisted Graphene Synthesis by Direct Exfoliation of Graphite Powder and its Cytotoxicity on SiHa Cell Line
Prabhat Kumar1, Purnima Jain1, Anjana Sarkar1★★ Corresponding author
- 1Department of Chemistry, Netaji Subhas University of Technology, New Delhi, INDIA.
CORRESPONDENCE
Anjana Sarkar
Department of Chemistry, Netaji Subhas University of Technology, New Delhi, INDIA.
Received: 10-03-2022; Revised: 07-05-2022; Accepted: 05-06-2022.
Volume 56, Issue 3 · pp. 732–738 · PUBLISHED 2022 · DOI: 10.5530/ijper.56.3.122
ABSTRACT
A cost-effective mass production of pristine graphene nanosheet is still an important challenge to harness its maximum potential in medical sciences. Here, we have used a binary solvent mixture of low dielectric constant (1,2,4- trichlorobenzene,ε = 2.24 and benzylamine,ε = 4.6) to produce a high quantity (0.6 mg/ml) of graphene under ultrasonication without compromising its quality. Obtained sample were characterized through XRD, UV-Vis, Raman, FT-IR, TEM-SAED, and cytopotency was evaluated on SiHa cell line. IG/I2D < 1.0 (~ 0.39) was achieved on more than 250 μm2 area, indicating the uniformity and obtained ID/IG ratio was 1.21. No toxicity was found in the case of 24 hr on the SiHa cell line. EC50 computation was 84.90 μg /ml for 48 hr and 62.03 μg /ml for 72 hr. Exfoliated graphene remained stable in binary solvent even after 1 year. The proposed experiment and obtained data can be powerful tools to explore the synergic effect of binary solvent exfoliation in combination to establish graphene as a biomaterial for neuronal network, tissue engineering, drug delivery, and regenerative medicines, to name a few.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Kumar, P., Jain, P., & Sarkar, A. (2022). Binary Solvent-assisted Graphene Synthesis by Direct Exfoliation of Graphite Powder and its Cytotoxicity on SiHa Cell Line. Indian Journal of Pharmaceutical Education and Research, 56(3), 732–738. https://doi.org/10.5530/ijper.56.3.122