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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Evaluation of Anti-cancer Activities of Cranberries Juice Concentrate in Osteosarcoma Cell Lines (MG-63)

Aishwarya Hattiholi1, Shivani Tendulkar2, Vijay Kumbar1, Malleswara Rao3, Manohar Kugaji1, Uday Muddapur4, Kishore Bhat1★★ Corresponding author

  1. 1Central Research Laboratory, Maratha Mandal’s Nathajirao G. Halgekar Institute of Dental Sciences and Research Center, Near KSRP Ground, Bauxite Road, Belgaum, Karnataka, INDIA.
  2. 2Dr. Prabhakar Kore Basic Science Research Center, KLE Academy of Higher Education and Research, Belgaum, Karnataka, INDIA.
  3. 3Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Guntur, Andra Pradesh, INDIA.
  4. 4Department of Biotechnology, KLE Technological University (Formerly known as B.V. Bhoomaraddi College of Engineering and Technology), BVB Campus, Hubli, Karnataka, INDIA.

CORRESPONDENCE

Kishore Bhat

Central Research Laboratory, Maratha Mandal’s Nathajirao G. Halgekar Institute of Dental Sciences and Research Center, Near KSRP Ground, Bauxite Road, Belgaum, Karnataka, INDIA.

drkgbhat@yahoo.com

Received: 23-10-2021; Revised: 07-05-2022; Accepted: 07-07-2022.

Volume 56, Issue 4 · pp. 1141–1149 · PUBLISHED 2022 · DOI: 10.5530/ijper.56.4.195

View on IJPER original site ↗

ABSTRACT

Aim/Background: Osteosarcoma is one of the prevalent cancers occurring mostly in adolescents and has a high risk of malignancy. With complications involved in the current treatment strategies, alternates including the use of phytochemicals have gained fame. Cranberries are known for their exceptional health benefits and have been explored for their effective activities in various cancers. The current study aimed at evaluating the anti-cancer properties of cranberry juice concentrate (CJC) on MG-63 cell line for human osteosarcoma, by investigating its apoptotic activity through changes in cell viability and mitochondrial membrane potential. Materials and Methods: Cranberry juice concentrate was obtained by pulverization and lyophilization. The MG-63 cells were treated with 12.5-800 μg/mL of the CJC and incubated for 24, 48, and 72 hr. The percentage cell viability and IC50 values were obtained. The mitochondrial membrane potential and nuclear changes were examined. The induction of apoptosis was studied by flow cytometer using BD cell Quest 7.5.3 software. GraphPad Prism was used for statistical analysis with significant p-value at <0.05. Results: The IC50 values obtained for CJC were 847.9, 637.4, and 440.6 μg/mL for 24, 48, and 72 hr respectively. Change in the mitochondrial membrane potential and nuclear morphology was observed following incubation with CJC. Flow cytometric analysis shows cells detected at early and late apoptoic stages after treatment with CJC. Conclusion: Our result suggests that CJC has significant effects on MG-63 osteosarcoma cells and can be considered to supplement conventional therapeutic strategies.

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    Hattiholi, A., Tendulkar, S., Kumbar, V., Rao, M., Kugaji, M., Muddapur, U., & Bhat, K. (2022). Evaluation of Anti-cancer Activities of Cranberries Juice Concentrate in Osteosarcoma Cell Lines (MG-63). Indian Journal of Pharmaceutical Education and Research, 56(4), 1141–1149. https://doi.org/10.5530/ijper.56.4.195