Troxerutin, a Bioflavonoid Exhibits Oxidative Stress-induced Cytotoxic Effects on KB Cells
Li Tan1, Huanping Ding2, Haimeng Xi1★★ Corresponding author
- 1School of Stomatology, Qilu Medical College, Renmin Xi Lu, Zibo City, Shandong Province, CHINA.
- 2Student Affairs Office, Qilu Medical College, Renmin Xi Lu, Zibo City, Shandong Province, CHINA.
CORRESPONDENCE
Haimeng Xi
School of Stomatology, Qilu Medical College, Renmin Xi Lu, Zibo City, Shandong Province, 255300, CHINA.
Received: 25-05-2022; Revised: 11-10-2022; Accepted: 18-11-2022.
Volume 57, Issue 1 · pp. 167–172 · PUBLISHED 2023 · DOI: 10.5530/001954642011
ABSTRACT
Background: The prevalence of oral cancer is high and it is the sixth most occurring cancer in the world. Some of the drugs used to treat oral cancer cause adverse effects and are resistant to treatment. Objectives: This study was designed and performed to evaluate the cytotoxic and anti-cancerous effects of troxerutin. Materials and Methods: Cell proliferation, cell viability, and morphological analysis were performed with different doses of troxerutin. Once the IC50 value was obtained, doses were fixed and nitric oxide and lipid peroxidation were measured. Qualitative analysis of ROS and mitochondrial membrane potential was performed using 2′,7′-dichlorofluorescein methods and rhodamine 123 fluorescent staining respectively. Results: Troxerutin caused cytotoxicity and reduced cell viability. The IC50 value was 50 μg/ml. Morphological analysis revealed KB cells suffered after troxerutin treatment. Nitric oxide and TBARS levels were elevated. ROS concentration increased and mitochondrial membrane potential diminished with a higher concentration of the drug. Conclusion: Troxerutin exhibits an anti-cancer effect and can act as a NO-donating and ROS-promoting compound aiding in chemotherapy.
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Tan, L., Ding, H., & Xi, H. (2023). Troxerutin, a Bioflavonoid Exhibits Oxidative Stress-induced Cytotoxic Effects on KB Cells. Indian Journal of Pharmaceutical Education and Research, 57(1), 167–172. https://doi.org/10.5530/001954642011