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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Binding of Novobiocin Paves the Way for Inhibition of DEAH-box Helicase 8

Mohammad Rehan Ajmal1★, Fahad Mohammad Almutairi1, Arif Hussain2, Mohammad Fahad Ullah3, M Ayaz Ahmad4,5, Ali Saber Abdelhameed6, Hussain Arif7★ Corresponding author

  1. 1Department of Biochemistry, Physical Biochemistry Research Laboratory, Faculty of Science, University of Tabuk, Tabuk, SAUDI ARABIA.
  2. 2School of Life Sciences, Manipal Academy of Higher Education-Dubai Campus, Dubai, UNITED ARAB EMIRATES.
  3. 3Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, SAUDI ARABIA.
  4. 4Department of Physics, Faculty of Science, University of Tabuk, SAUDI ARABIA.
  5. 5Department of Mathematics, Physics and Statistics, University of Guyana, South America, 521 Good Hope Coast Demerara, Georgetown, GUYANA.
  6. 6Department of Pharmaceutical Chemistry, College of Pharmacy, King Saudi University, Riyadh, SAUDI ARABIA.
  7. 7Department of Biochemistry, Mohammad Ali Jauhar University, Rampur, Uttar Pradesh, INDIA.

CORRESPONDENCE

Mohammad Rehan Ajmal

Department of Biochemistry, Physical Biochemistry Research Laboratory, Faculty of Science, University of Tabuk, Tabuk-71491, SAUDI ARABIA.

mkhan@ut.edu.sa

Received: 22-02-2023; Revised: 03-04-2023; Accepted: 05-05-2023.

Volume 57, Issue 2S · pp. s459–s464 · PUBLISHED 2023 · DOI: 10.5530/ijper.57.2s.54

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ABSTRACT

Introduction: DEAH-box helicase 8 (DHX8) is a crucial DEAH-box RNA helicase involved in splicing and required for the release of mature mRNA from the splice. Here, we report the interaction study of human DHX8 and four test compounds namely etoposide, netropsin, nogalamycin, and novobiocin. Materials and Methods: Molecular docking and fluorescence emission spectra techniques were employed to determine the binding and inhibitory effect of test compounds. Results: Our docking and fluorescence emission spectra results showed that DHX8 has a good binding preference for novobiocin among these four test compounds. Moreover, fluorescence emission spectra of DHX8 with novobiocin also revealed the 2.5 µM concentration is an effective novobiocin concentration to inhibit the activity of DHX8. Conclusion: These findings provide an in-depth understanding of the inhibition of DHX8 and contribute insights towards the development of novobiocin as a therapeutic molecule against the DHX8 in targeted diseases.

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    Ajmal, M. R., Almutairi, F. M., Hussain, A., Ullah, M. F., Ahmad, M. A., Abdelhameed, A. S., & Arif, H. (2023). Binding of Novobiocin Paves the Way for Inhibition of DEAH-box Helicase 8. Indian Journal of Pharmaceutical Education and Research, 57(2S), s459–s464. https://doi.org/10.5530/ijper.57.2s.54