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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Stability Indicating Reverse Phase-High Performance Liquid Chromatography Method for Simultaneous Estimation of Cabotegravir and Rilpivirine

Padmabhushana Chary Vemuluri1,2★, Sireesha Dodda1★ Corresponding author

  1. 1Department of Pharmaceutical Analysis, Anurag University, Hyderabad, Telangana, INDIA.
  2. 2Department of Pharmaceutical Analysis, Anurag Pharmacy College, Kodad, Telangana, INDIA.

CORRESPONDENCE

Padmabhushana Chary Vemuluri

Mr. V. Padmabhushana Chary,Assistant professor, Department of Pharmaceutical Analysis, Anurag Pharmacy College, Kodad-508206, Telangana, INDIA.

21ph304104@anurag.edu.in

Received: 25-08-2022; Revised: 18-01-2023; Accepted: 01-04-2023.

Volume 57, Issue 3S · pp. s766–s771 · PUBLISHED 2023 · DOI:

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ABSTRACT

Background: For the estimation of cabotegravir and rilpivirine in the bulk and pharmaceutical dosage form, a stability-indicating reverse-phase high-performance liquid chromatography method was developed and validated using an inertsil C18 (150 x 4.6 mm, 5 µm) column. At a flow rate of 1.0 ml/min, a mobile phase containing a mixture of 0.01N ammonium acetate buffer (pH 3) and acetonitrile (65:35, v/v) was passed over the column. The column temperature was set at 30°C. A photodiode array detector was used at the wavelength of 257 nm. Results: Retention times of cabotegravir and rilpivirine were found to be 2.250 min and 2.823 min, respectively. The calibration curves were linear over the concentration range of 10-60 µg/ml and 15-90 µg/ml for cabotegravir and rilpivirine, respectively with a correlation coefficient (R2) of 0.999. The percent relative standard deviation (% RSD) for precision and robustness studies was found to be < 2%. The mean % recovery was obtained as 100.71 % and 100.01 % for cabotegravir and rilpivirine, respectively. The degradation during stability studies was more in the presence of oxidative conditions. Conclusion: The developed method was found to be simple, rapid, and economical and can be applied successfully for simultaneous estimation of cabotegravir and rilpivirine in regular analysis.

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    Vemuluri, P. C., & Dodda, S. (2023). Stability Indicating Reverse Phase-High Performance Liquid Chromatography Method for Simultaneous Estimation of Cabotegravir and Rilpivirine. Indian Journal of Pharmaceutical Education and Research, 57(3S), s766–s771.