Partially Purified Polysaccharides from Lentinus edodes (Mushroom) Scavenge Free Radicals and Induce Apoptosis in MCF-7 Cancer Cells by Regulating Apoptotic Genes

ABSTRACT

Background: Therapeutic products obtained from nature have been gaining attention for health improvement, disease treatment, and drug development. Traditional therapeutic compounds from plants, animals, and microorganisms and their bioactive constituents have led to the development of therapeutics for various diseases, including cancer. Mushrooms are widely consumed products because of their nutritional and therapeutic potential. Materials and Methods: This study aimed to extract partially purified polysaccharides from Lentinus edodes (PLE) to a study to identify bioactive compounds by GC-MS and HPLC. Furthermore, we examined PLE to identify its antioxidant potential and effect on breast cancer cells (MCF-7 cell lines). Results: GC-MS revealed the presence of 45 bioactive components from PLE, and HPLC analysis showed the presence of β-glucan. Furthermore, PLE showed potential antioxidant activity, which was confirmed by significant free radical (DPPH, FRAP, and ABTS) scavenging and total antioxidant activity analysis. In addition, PLE showed noteworthy anticancer activity, substantiated by decreased cell viability in a dose-dependent manner (100, 200, 300, and 400μg/ mL) manner. On the other hand, RT-PCR analysis revealed that PLE potentially up-regulated caspase-3, toll-like receptor 4, and Bcl-2-associated X protein and concurrently down-regulated B-cell lymphoma 2 (Bcl-2), which confirmed that PLE can induce apoptosis in MCF-7 cancer cells. Conclusion: Hence, this study revealed that PLE can induce apoptosis in cancer cells and fight against breast cancer through its antioxidant potential. These results recommend that PLE might be considered a nutraceutical for managing breast cancer.

KEYWORDS

• Mushrooms
• Nutraceuticals
• Breast Cancer
• Apoptosis
• Antioxidants

Showing references and in-text citations exactly as published.

• DOI10.5530/ijper.58.2.60Copy

RELATED ARTICLES

Matched on section and article type within this issue of IJPER.

• Article · VOLUME 58, ISSUE 2Evaluation of Student Performance Based on Integrated Teaching Method in Pharmacology CurriculaYulin Zhang, Jia Liu, Wei Xu, Qin Kong · pp. 411–416Original ArticleSame type: Article
• Article · VOLUME 58, ISSUE 2Pulsatile Drug Delivery Systems of Esomeprazole: Optimization through Quality by DesignPhaneendra Kurapati, Santhivardhan Chinni · pp. 417–431Original ArticleSame type: Article
• Article · VOLUME 58, ISSUE 2Fabrication of Topical Pluronic Lecithin Organogel Containing Immunomodulatory Drug for Site-Specific Drug DeliveryDipti Gohil, Miten Shah, Nirmal Shah, Rajesh A. Maheshwari · pp. 432–440Original ArticleSame type: Article
• Article · VOLUME 58, ISSUE 2Development and Evaluation of Fenofibrate Surface Solid Dispersion for Improved Solubility and Dissolution RateAvinash Hanmant Hosmani, Nikhil Dilip Patil, Arti Achut Pawar, Sandip Mohan Honmane, Yogesh Shripad Thorat · pp. 446–452Original ArticleSame type: Article