An Exploration of Chrysin Fabricated Silver Nanoparticles as Antibiofilm Agent against Pseudomonas aeruginosa
Arnica F Lal, Pushpraj S Gupta★★ Corresponding author
- 1Department of Pharmaceutical Science, SHALOM Institute of Health and Allied Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Naini, Prayagraj, Uttar Pradesh, INDIA.
CORRESPONDENCE
Pushpraj S Gupta
Department of Pharmaceutical Science, SHALOM Institute of Health and Allied Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Naini, Prayagraj-211007, Uttar Pradesh, INDIA.
Received: 26-05-2023; Revised: 09-10-2023; Accepted: 19-01-2024.
Volume 58, Issue 2S · pp. s429–s435 · PUBLISHED Apr-Jun 2024 · DOI: 10.5530/ijper.58.2s.46
ABSTRACT
Background Pseudomonas aeruginosa (P. aeruginosa) is the source of serious nosocomial infections, the most common of which is ventilator-associated pneumonia. P. aeruginosa infections continue to pose a substantial therapeutic problem. The expression of several virulence genes and the formation of biofilms in bacteria are caused by quorum sensing, a density-dependent cell-to-cell communication mechanism. Anti-biofilm chemicals prevent the synthesis of the polymer matrix, limit cell adhesion and attachment, reduce the generation of virulence factors, and obstruct the quorum sensing system. The present study focused on the antibiofilm activity of chrysin-fabricated silver nanoparticles (nano-chrysin) against P. aeruginosa. Materials and Methods In our study, chrysin, which is a polyphenol, was fabricated with silver nanoparticles to make nano-chrysin. P. aeruginosa PAO1 strain was treated at sub-MIC concentrations with chrysin (50, 25, 12.5, and 6.25 μg mL-1), silver nanoparticles (6.26, 3.13, 1.56, and 0.78 μg mL-1) and formulated nano-chrysin (3.13, 1.56, 0.78, and 0.39μg mL-1) to find out the effectiveness of these compounds against biofilm formation. Results Biofilm produced by P. aeruginosa PAO1 was found to be inhibited at sub-MIC concentrations (3.13, 1.56, 0.78, and 0.39 μg mL-1) of nano-chrysin having MIC value ranging between 50-3.13 μg mL-1 which is more potent than alone chrysin and silver nanoparticles. Conclusion The data confirmed that nano-chrysin is effective in inhibiting biofilm formation, produced by P. aeruginosa.
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Lal, A. F., & Gupta, P. S. (2024). An Exploration of Chrysin Fabricated Silver Nanoparticles as Antibiofilm Agent against Pseudomonas aeruginosa. Indian Journal of Pharmaceutical Education and Research, 58(2S), s429–s435. https://doi.org/10.5530/ijper.58.2s.46