Method Development and Validation of Antihypertensive Drugs Using HPLC Technique

ABSTRACT

Aim A validated RP-HPLC method was established for the quantification of Amlodipine and Metoprolol succinate according to ICH guidelines. Materials and Methods This method utilized a Phenomenex (US) C18 column with dimensions of 250X4.6 mm I.D and a particle size of 5 µm. Employing an isocratic elution technique, the mobile phase comprised pH 3.0 phosphate buffer solution and acetonitrile. Detection occurred at a wavelength of 215 nm and a flow rate of 1 mL/min was chosen to ensure optimal resolution for Amlodipine and Metoprolol succinate. Results and Discussion Retention times were noted at 3.137 min and 5.672 min for Amlodipine and Metoprolol succinate, respectively. High correlation coefficients were achieved (0.9995 for Amlodipine and 0.9996 for Metoprolol succinate). The method exhibited precision, with low relative standard deviations of 0.357% for Amlodipine and 0.077% for Metoprolol succinate. In summary, the RP-HPLC method developed was specific, linear, precise and robust. Conclusion The devised method was validated in terms of accuracy, precision, linearity, specificity, robustness and ruggedness. All formulations’ sample recoveries were in good accordance with the claims made on their labels

KEYWORDS

• Amlodipine
• Metoprolol succinate
• Retention time
• Correlation coefficient
• RP-HPLC

Showing references and in-text citations exactly as published.

• DOI10.5530/ijper.58.3s.105Copy

RELATED ARTICLES

Matched on section and article type within this issue of IJPER.

• Article · VOLUME 58, ISSUE 3SLow Dose Lipopolysaccharide-Induced Depressive-Like Phenotype is Mediated by Proinflammatory Cytokines in Mice and Role of KetamineShuvranshu Praharaj, Vandurayanpet Kaliyamoorthy Kalaichelvan, Vedigounder Murugan, Velappan Venkatachalam, Ishtiyaque Ahmad · pp. s777–s786Original ArticleSame type: Article
• Article · VOLUME 58, ISSUE 3SInterstitial Lung Diseases Classification in the Context of Pharmaceutical Education and Research: A Two-Level Deep Learning ApproachVanita Dnyandev Jadhav, Lalit Vasantrao Patil · pp. s787–s795Original ArticleSame type: Article
• Article · VOLUME 58, ISSUE 3SComputational Study and Evaluation of Lidocaine and Nitroglycerin Ointment for External Hemorrhoid TreatmentArwa Alshargabi, Wafa Mohammed Al-Madhagi, Abdulkarim Kassem Alzomor · pp. s796–s804Original ArticleSame type: Article
• Article · VOLUME 58, ISSUE 3SSmart Manipulated UV Spectroscopic Methods for Resolving the Overlapped Spectra for Quality Control of Two Analgesic Binary Combination FormulationsMahesh Attimarad, Bandar Aldhubiab, Sreeharsha Nagaraja, Shinu Pottathil · pp. s796–s804Original ArticleSame type: Article