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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Development and Validation of a HPLC-PDA Method to Quantification of Ketobemidone in Rat Plasma and its’ Application in Pharmacokinetic Study

Shashipal Aitha1★, Subramani Devaraju3★, Jayanti Naga Sri Rama Chandra Murthy2★, Kiran Kumar Tatapudi2★, Leela Prasad Geddada2★, Tiruveedhula Somasekhar4★, Hemambika Sadasivuni4★, Muralidharan Kaliyaperumal2★★ Corresponding author

  1. 1Department of Chemistry, School of Applied Science and Humanities, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Guntur, Andhra Pradesh, India.
  2. 2Discovery Analytical R and D Department, Aragen Life Sciences Pvt. Ltd., IDA Nacharam, Hyderabad, Telangana, INDIA.
  3. 3Polymer Engineering Laboratory, Department of Chemistry, PSG Institute of Technology and Applied Research, Neelambur, Coimbatore, Tamil Nadu, INDIA.
  4. 4Department of Freshmen Engineering, St. Martin’s Engineering College, Dhulapally, Hyderabad, Telangana, INDIA.

CORRESPONDENCE

Shashipal Aitha

Dr. Subramani DevarajuDepartment of Chemistry, School of Applied Science and Humanities, Vignan's Foundation for Science, Technology and Research, Vadlamudi, Guntur-522213, Andhra Pradesh, INDIA.

subudeva@gmail.com

Received: 16-05-2024; Revised: 05-09-2024; Accepted: 02-01-2025.

Volume 59, Issue 2S · pp. s740–s747 · PUBLISHED Apr-Jun 2025 · DOI: 10.5530/ijper.20256924

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ABSTRACT

Aim: Ketobemidone is an opioid drug that have been using against pain in various circumstances. The current research was aimed to develop and validate a HPLC-PDA method to estimate the ketobemidone in rat plasma. Materials and Methods: The ketobemidone in the biological matrices (rat plasma) was separated using solvent extraction method using methanol as extracting solvent. An isocratic mobile phase consisting of methanol-water-phosphate buffer at 40:60:0.01 % v/v/v and Kromasil C18 column were used for separation in Liquid Chromatography (LC). Results: The selected linearity range i.e., 0.10-25μg/mL was acceptable with r2 value 0.9998 as least square method. The determined Lower Limit of Quantification (LLOQ) is 0.10 μg/mL. The intermediate precision studies were performed within and between days; and the data obtained are within a significant range, i.e., %RSD <9.2 and the accuracy was within 10.0% of the relative error. Conclusion: The developed method to detect the ketobemidone was successfully validated and it was successfully applied on rat pharmacokinetic study.

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    Aitha, S., Devaraju, S., Murthy, J. N. S. R. C., Tatapudi, K. K., Geddada, L. P., Somasekhar, T., Sadasivuni, H., & Kaliyaperumal, M. (2025). Development and Validation of a HPLC-PDA Method to Quantification of Ketobemidone in Rat Plasma and its’ Application in Pharmacokinetic Study. Indian Journal of Pharmaceutical Education and Research, 59(2S), s740–s747. https://doi.org/10.5530/ijper.20256924