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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Development and Optimization of Novel Microwave-Assisted Extraction for Rich-Flavonoids and Polyphenols from Dioscorea bulbifera Leaves by Response Surface Methodology

Soma Uday Kumar Reddy1, Divya Jyothi1★, Krishna Kana Veetil1, Shreya Shreya1, Rokeya Sultana2, Sneh Priya3★ Corresponding author

  1. 1Department of Pharmacognosy, NGSM Institute of Pharmaceutical Sciences, NITTE (Deemed to be University), Deralakatte, Karnataka, INDIA.
  2. 2Department of Pharmacognosy, Yenepoya Pharmacy College, Yenepoya (Deemed to be University), Deralakatte, Karnataka, INDIA.
  3. 3Department of Pharmaceutics, NGSM Institute of Pharmaceutical Sciences, NITTE (Deemed to be University), Deralakatte, Karnataka, INDIA.

CORRESPONDENCE

Divya Jyothi

Department of Pharmacognosy, NGSM Institute of Pharmaceutical Sciences, NITTE (Deemed to be University), Deralakatte, Karnataka, INDIA.

divyajyothi@nitte.edu.in

Received: 29-11-2024; Revised: 07-02-2025; Accepted: 15-05-2025.

Volume 59, Issue 4 · pp. 1357–1365 · PUBLISHED 2025 · DOI: 10.5530/ijper.20250520

View on IJPER original site ↗

ABSTRACT

Objectives: Microwave-Assisted Extraction (MAE) has emerged as a promising technique for the extraction of bioactive compounds from plant materials due to its efficiency and reduced processing time. In the present study, variables of MAE were optimized for maximizing the extraction of polyphenolic and flavonoid constituents from Dioscorea bulbifera leaves by Box-Behnken Design (BBD) of response surface methodology. Materials and Methods: Box-Behnken Design (BBD) was utilized to find the optimal microwave extraction condition for obtaining extracts enriched with polyphenolic compounds. The independent variables for BBD are irradiation power (400-800W), time (1-5 min) and concentration of ethanol (20-60%). Total phenolic content and total flavonoid content were estimated by the Folin ciocalteu method and aluminium chloride colorimetric assay respectively. Using qualitative chemical testing and FTIR analysis, the phytoconstituents and functional properties of the extracts were determined. Results: The ANOVA results obtained from the design of expert software exhibited that Total Phenolic Content (TPC) and total flavonoid content were significantly (p<0.0001) affected by microwave power of irradiation, time and concentration of ethanol, indicating good agreement between the experiment and predicted values. The ideal parameter of MAE for maximum yield of TPC (3.49 mg GAE/g) and TFC (0.61 mg QE/g) were 400W of microwave power, 5 min of irradiation time and 48.467% ethanol concentration. Conclusion: The optimized extracts can be used as a source of antioxidants with potential applications in functional foods, dietary supplements and pharmaceutical formulations aimed at combating oxidative stress-related diseases.

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    Reddy, S. U. K., Jyothi, D., Veetil, K. K., Shreya, S., Sultana, R., & Priya, S. (2025). Development and Optimization of Novel Microwave-Assisted Extraction for Rich-Flavonoids and Polyphenols from Dioscorea bulbifera Leaves by Response Surface Methodology. Indian Journal of Pharmaceutical Education and Research, 59(4), 1357–1365. https://doi.org/10.5530/ijper.20250520