Method Development and Validation for the Simultaneous Estimation of Aflatoxins in Botanical Origin Samples Using High-Performance Thin-Layer Chromatography

ABSTRACT

Background and Objectives: Aflatoxins are toxic secondary metabolites produced by certain Aspergillus species and are frequently found in contaminated food and herbal products. Their presence poses serious health risks, necessitating effective analytical methods for detection and control. In this study, a simple, sensitive, and cost-effective High-Performance Thin-Layer Chromatography (HPTLC) method was developed and validated for the simultaneous estimation of aflatoxins B₁, B₂, G₁, and G₂ in a polyherbal formulation, Haridrakhand. Materials and Methods: The method employed silica gel 60 F₂₅₄ plates and a mobile phase of chloroform: acetone: water (140:20:0.3, v/v/v), with detection at 366 nm under UV light. The method was validated as per ICH Q2(R2) guidelines for linearity, accuracy, precision, specificity, robustness, Limit of Detection (LOD), and Limit of Quantification (LOQ). Results: Calibration curves showed excellent linearity with Correlation Coefficients (R²) greater than 0.996. Recovery studies at 80%, 100%, and 120% levels yielded recovery rates within 98-102%. The LOD and LOQ values confirmed the method’s sensitivity, and all validation parameters met the required acceptance criteria. Conclusion: This HPTLC method is suitable for routine screening and quality control of aflatoxins in herbal formulations.

KEYWORDS

• Botanical Sample
• Liquid Chromatography
• Method Development
• Validation
• Toxins

Showing references and in-text citations exactly as published.

• DOI10.5530/ijper.20265177Copy

RELATED ARTICLES

Matched on section and article type within this issue of IJPER.

• Article · VOLUME 60, ISSUE 4SFormulation and Evaluation of Liquisolid Tablet with Enhanced Dissolution of Ivacaftor by Using Box-Behnken DesignManisha Dnyanoba Chate, Swaroop Rameshwarji Lahoti · pp. s1405–s1415Original ArticleSame type: Article
• Article · VOLUME 60, ISSUE 4SDevelopment and Evaluation of Polymer-conjugated Solid Fast Dissolving Tablets of Ambroxol HydrochlorideVidhan Chand Bala, Mukesh Kumar Singh, Amit Kumar, Arvind Kumar Patel, Sunil Kumar Tiwari, Asheesh Kumar Gupta, Sushil Kumar · pp. s1416–s1429Original ArticleSame type: Article
• Article · VOLUME 60, ISSUE 4SQuality by Design Development of a Thermosensitive in situ Gel for Enhanced Ocular Delivery of CarvedilolAnurag Rajendra Panade, Sandip Akaram Bandgar, Riya Raghunath Patil, Indrayani Sandip Bandgar · pp. s1430–s1444Original ArticleSame type: Article
• Article · VOLUME 60, ISSUE 4SNovel Application of Mixed Solvency Concept for the Development of Topical Emulgel Formulations of Poorly Water-Soluble Drug, Piroxicam, and their EvaluationsJitendra Singh, Prateek Sahu, Sharad Prakash Pandey, Rajesh Kumar Maheshwari · pp. s1445–s1460Original ArticleSame type: Article