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International Journal of Pharmaceutical InvestigationNOW IN
Article

GC-MS Profiling, in vitro Antioxidant and Antimicrobial Activities of Kaempferia parviflora Wall. ex. Baker Rhizome Extract

Rameshor Singh Atom1,2★, Shaukat Ali M Shaikh3,4, Kishorjit Maharabam2, Rakhi Khunjamayum5, Dini Ahanthem2, Warjeet Singh Laitonjam2, Raghumani Singh Ningthoujam6, Amit Kunwar7★ Corresponding author

  1. 1Department of Chemistry, Waikhom Mani Girls’ College, Thoubal, Manipur, INDIA.
  2. 2Department of Chemistry, Manipur University, Imphal, Manipur, INDIA.
  3. 3School of Chemical Sciences, Centre for Excellence in Basic Sciences, Mumbai University, Mumbai, Maharashtra, INDIA.
  4. 4Department of Atomic Energy, Mumbai University, Mumbai, Maharashtra, INDIA.
  5. 5Department of Biochemistry, Manipur University, Imphal, Manipur, INDIA.
  6. 6Chemistry Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, INDIA.
  7. 7Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, INDIA.

CORRESPONDENCE

Rameshor Singh Atom

Department of Chemistry, Waikhom Mani Girls’ College, Thoubal, Manipur, INDIA.

rameshoratom@gmail.com

Received: 12-06-2022; Revised: 06-08-2022; Accepted: 27-08-2022.

Volume 12, Issue 4 · pp. 430–437 · PUBLISHED · DOI: 10.5530/ijpi.2022.4.74

View on IJPI original site ↗

ABSTRACT

Bakground: Kaempferia parviflora (zingiberaceae family) rhizomes are used for various pharmacological applications. The present study is aimed for putative identification of phyto-constituents in the chloroform extract of the rhizome of Kaempferia parviflora and to correlate with bioactivities like antioxidant and anti-microbial/fungal activities. Materials and Methods:Rhizome extract was prepared using Soxhlet extractor and undergone the phytoconstituents analysis of it by gas chromatography–mass spectrometry (GC-MS). Results:Identified twenty-five compounds, out of which 4H-1-Benzopyran- 4-0ne,5,7-dimethoxy-2-phenyl and Hydroxyurea showed the maximum and minimum relative abundance of 76.4% and 0.002% respectively. By agar well diffusion method, the extract (25 μg/ml) exhibited highest antibacterial potential against Micrococcus luteus (MTCC 106) (gram-positive bacteria) and anti-fungal potential against plant pathogen Rhizoctonia solani (MTCC 4633). The extract also showed the potent scavenging activities of 2,2-diphenyl-1-picryl-hydrazine (DPPH) and 2,2′-Azino-bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) radicals with IC50 values of 3.0 ± 0.1 mg/ml and 19.4 ± 1.2 μg/ml respectively. Subsequently, lipid peroxidation assay was also used for estimation of antioxidant activity of this extract. The extract (20 μg/ml) inhibited lipid peroxidation by 80.0 ± 4.5%. Conclusion:The outcomes of present study provide scope for isolation of bioactive compounds from Kaempferia parviflora for medicinal application.

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    Atom, R. S., Shaikh, S. A. M., Maharabam, K., Khunjamayum, R., Ahanthem, D., Laitonjam, W. S., Ningthoujam, R. S., & Kunwar, A. (). GC-MS Profiling, in vitro Antioxidant and Antimicrobial Activities of Kaempferia parviflora Wall. ex. Baker Rhizome Extract. International Journal of Pharmaceutical Investigation, 12(4), 430–437. https://doi.org/10.5530/ijpi.2022.4.74