Polymeric Nanocarrier system Bearing Anticancer Agent for the Treatment of Prostate Cancer: Systematic Development and in vitro Characterization

ABSTRACT

Objectives:Doxorubicin-bearing polymeric (PLGA) nanoparticles (PNPs) were prepared for the treatment of prostate cancer. Materials and Methods:These PNPs were prepared using the solvent evaporation method and characterized using UV, NMR, Particle size Analyser, SEM, and TEM for the determination of shape, size, zeta potential, and polydispersity index. Moreover, in vitro drug release, SRB assay, apoptosis, and haemolytic study were also performed to prove its potentiality for prostate cancer. Results:The mean particle size (MPS), polydispersity index (PDI), and zeta potential (ZP) of PNPs were found to be 101.3 ± 1.23 nm, 0.240 ± 0.28 and -3.11 ± 1.96 mV, respectively. SEM and TEM revealed that the PNPs are spherical in shape and the sizes are approximately 100 nm. The entrapment efficiency, loading efficiency, and percentage drug release of doxorubicin from PNPs were found to be 69.38 ± 1.76%, 4.2 ± 0.64% and 77.56 ± 4.24%, respectively. In addition, cellular apoptosis against PC-3 cell lines was found to be ≥ 5.15 fold and haemolysis toxicity was reduced to ≤ 3.5 fold with PNPs as compared to free drug. Conclusion: These findings demonstrated that PNPs have the potential to deliver the anticancer agent to tumor sites and could be an emerging strategy for the treatment of prostate cancer.

KEYWORDS

• Polymeric nanoparticles
• Targeting
• Doxorubicin
• PLGA
• Prostate cancer

Showing references and in-text citations exactly as published.

• DOI10.5530/223097131799Copy

RELATED ARTICLES

Matched on section and article type within this issue of IJPI.

• Article · VOLUME 13, ISSUE 1Cardioprotective and Hepatoprotective Effects of Ixora pavetta and Tecoma capensis Leaf ExtractsDara Suneeta, YRKV Tirupathi Rao · pp. 22–27Original ArticleSame type: Article
• Article · VOLUME 13, ISSUE 1Optimization of Green Synthesized Black Tea Nanoparticles using Central Composite DesignSarika Nikam, Shilpa Chaudhari · pp. 28–36Original ArticleSame type: Article
• Article · VOLUME 13, ISSUE 1Prospective Study on Identification of Risk Factors, Assessment of Respiratory Distress in PediatricsMachavarapu Neelima, Yekkala Prathyusha, Mamindla Archana, Thirunagiri Praveen kumar · pp. 37–42Original ArticleSame type: Article
• Article · VOLUME 13, ISSUE 1Age-related Variation in TSH Levels in Patients with Down’s Syndrome -A Ten Year Longitudinal StudyMandar Ambike, Nabilah Kazi, Arun Prasad, Dnyandeo Chopade, Suresh Kondaveeti · pp. 43–47Original ArticleSame type: Article