jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
International Journal of Pharmaceutical InvestigationNOW IN
Article

Simultaneous Estimation of Gallic Acid, Vasicine, Pterostilbene and Piperine Using RP-HPLC Method from Ayurvedic Proprietary Formulations

Sanjay U Nipanikar1★, Pravin S Surwade2, Ankita R Kasabe3, Priyanka B Patil3, Sachin A Upasani4★ Corresponding author

  1. 1MD (Ayu) Chief Technical Officer (CTO), Ari Healthcare Pvt. Ltd., Kharadi, Pune, Maharashtra, INDIA.
  2. 2(Quality Assurance) Executive-Analytical, Ari Healthcare Pvt. Ltd., Kharadi, Pune, Maharashtra, INDIA.
  3. 3(Quality Assurance) Research Associate, Ari Healthcare Pvt. Ltd., Kharadi, Pune, Maharashtra, INDIA.
  4. 4(Pharmaceutical Medicine), Assistant General Manager (AGM)-Technical, Ari Healthcare Pvt. Ltd., Kharadi, Pune, Maharashtra, INDIA.

CORRESPONDENCE

Sanjay U Nipanikar

MD (Ayu) Chief Technical Officer (CTO), Ari Healthcare Pvt. Ltd., Kharadi, Pune, Maharashtra, INDIA.

sanjay.n@arihealthcare.in

Received: 21-04-2023; Revised: 01-05-2023; Accepted: 01-06-2023.

Volume 13, Issue 3 · pp. 656–665 · PUBLISHED · DOI: 10.5530/ijpi.13.3.082

View on IJPI original site ↗

ABSTRACT

Introduction: A simple, accurate, precise, sensitive, and validated RP-HPLC method was developed for simultaneous estimation of Gallic Acid (GA), Vasicine (VS), Pterostilbene (PTS) and Piperine (PP) from Ayurvedic Proprietary Formulations developed in two different dosage forms viz. Amarantha Gynorite syrup and Amarantha Gynorite capsule. Materials and Methods:The optimized chromatographic condition was used for separation on Agilent Zorbax SB-C18 (5 µm 4.6 x 250 mm), Rheodyne injector with volume of 20 µL and mobile phase consisting of Solvent A (70 percent methanol) and solvent B [10 nm Hexane sulfonic acid, Acetonitrile and Glacial Acetic acid (70:30:1)] in composition of 95: 05 v/v with isocratic elution for 40 min at flow rate of 0.5 mL/ min. Quantification was carried out using a photodiode array detector at 280 nm. The method was validated in accordance with International Conference on Harmonization (ICH) guidelines. Results and Conclusion:No chromatographic interference from syrup and capsule excipients was analyzed. Hence, the present analytical method is applicable for simultaneous determination of GA, VS, PTS and PP in Ayurvedic proprietary formulations.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Nipanikar, S. U., Surwade, P. S., Kasabe, A. R., Patil, P. B., & Upasani, S. A. (). Simultaneous Estimation of Gallic Acid, Vasicine, Pterostilbene and Piperine Using RP-HPLC Method from Ayurvedic Proprietary Formulations. International Journal of Pharmaceutical Investigation, 13(3), 656–665. https://doi.org/10.5530/ijpi.13.3.082