Novel Antibacterial Activity and Time Kill Kinetics of Ethanolic Extract of Ipomea marginata against Select Oral Micro-organisms Causing Gingivitis-An in vitro Study

ABSTRACT

Background:Leaves of the plant Ipomea marginatais commonly used in Indian folklore medicine to treat skin infections however the antibacterial property of Ipomea marginataagainst oral microorganisms causing gingivitis and periodontitis has not been explored till date. Hence, the present study is planned to compare and evaluate the antibacterial efficacy of ethanolic extract of Ipomea marginatawith 0.2% chlorhexidine mouth wash on select oral microorganisms. Materials and Methods:Phytochemical screening of ethanolic extract of leaves of Ipomea marginatashowed significant levels of secondary metabolites accountable for the antibacterial property of the leaves. Identification of the volatile constituents of the leaves of Ipomea margiantawas carried out using GC-MS and nine volatile compounds were identified. Antibacterial activity of ethanolic extract of Ipomea marginatawas evaluated against S. mutans, T. denticola, T. forsythiaand P. gingivaliswhich are the chief organisms indicated in the etiology of plaque induced gingivitis. The zone of inhibition and minimum inhibitory concentration was determined by disc diffusion and broth dilution method respectively and compared with gold standard 0.2% chlorhexidine. Time kills kinetics of test pathogens at 6, 12, 18 and 24 hr was also determined. The herbal extract exhibited statistically significant antibacterial property compared to standard chlorhexidine by agar disc diffusion method. Results:The Minimum Inhibitory Concentration (MIC) for the herbal extract for S. mutans, P. gingivalis, T. denticolaand T. forsythiawas 6.25 μg/ mL, 100 μg/mL, 100 μg/mL and 50 μg/mL, respectively and for 0.2% chlorhexidine it was 25 μg/ mL, 12.5 μg/mL, 200 μg/mL and 100 μg/mL respectively. Time kills kinetics revealed bactericidal activity of ethanolic extract of Ipomea marginataagainst oral microorganisms causing gingivitis in a time dependent manner. Conclusion:Ethanolic extract of leaves of Ipomea marginatashowed promising antibacterial activity compared to standard 0.2% chlorhexidine mouth wash.

KEYWORDS

• Antibacterial agent
• Cytotoxic
• Mouth wash

Showing references and in-text citations exactly as published.

• DOI10.5530/jyp.2024.16.30Copy

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