Synthesis, characterisation and evaluation of N-mannich bases of 2-substituted Benzimidazole derivatives
Sekar Vinoth Kumar2★, Mohan Raj Subramanian2, Santhosh Kumar Chinnaiyan3★ Corresponding author
- 1Department of Pharmaceutical Chemistry, Gokulakrishna College of Pharmacy, Andhra Pradesh 524121, INDIA.
- 2Department of Pharmacology, SSM College of Pharmacy, Tamil Nadu 638312, INDIA.
- 3Department of Pharmaceutical Biotechnology, Srikrupa Institute of Pharmaceutical Sciences, Andhra Pradesh 502277, INDIA.
CORRESPONDENCE
Sekar Vinoth Kumar
Department of Pharmaceutical Chemistry, Gokulakrishna College of Pharmacy, Andhra Pradesh 524121, INDIA.
Received: 23-08-2013; Revised: 16-11-2013; Accepted: 11-12-2013.
Volume 5, Issue 4 · pp. 154–159 · PUBLISHED · DOI: 10.1016/j.jyp.2013.11.004
ABSTRACT
Rationale:Benzimidazoles and its derivatives represent one of the mainly biological active classes of literature. Aim:In this present study aimed to synthesize N-mannich bases derivatives compounds bearing of 2-substituted benzimidazole moiety, in order to investigate their possible biological activity. Method:Benzimidazole compounds were prepared from the condensation reaction between ortho phenylene diamine and various acids. Mannich base of newly synthesized Benzimidazole derivatives were synthesized from 2-substituted Benzimidazoles by reacting with secondary amines. The purity of the compounds was ascertained by melting point (m.p) and thin layer chromatography (TLC). Structures of the synthesized compounds were elucidated by spectral data. Antimicrobial assay was performed by microbroth dilution method. Bacterial genomic DNA cleavage was assessed by Agarose gel electrophoresis. Toxicity of the most effective compounds was studied by Brine-shrimp lethality assay. Result:Among the synthesized compounds, compound 5E (a) and (b) was establish to be the most potent against all tested microorganisms. This two compounds exhibited complete bacterial DNA cleavage and non-toxic. Conclusion: These results suggest that it an interesting compound compared to the current therapeutic agents and are considered to investigate further for the same.
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Kumar, S. V., Subramanian, M. R., & Chinnaiyan, S. K. (). Synthesis, characterisation and evaluation of N-mannich bases of 2-substituted Benzimidazole derivatives. Journal of Young Pharmacists, 5(4), 154–159. https://doi.org/10.1016/j.jyp.2013.11.004