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Communication

Isolation, biochemical and genetic characterizations of alcohol-producing yeasts from the flowers of Woodfordia fruticosa

Jagdish Manwar1★, Kakasaheb Mahadik1, Anant Paradkar2, Lohidasan Sathiyanarayanan1, Mustafa Vohra3, Sanjay Patil3★ Corresponding author

  1. 1Poona College of Pharmacy, Bharati Vidyapeeth University, Pune 411 038, INDIA.
  2. 2Centre for Pharmaceutical Engineering Science, University of Bradford, Bradford BD7 1DP, UK.
  3. 3Department of Alcohol Technology, Vasantdada Sugar Institute, Pune 412 307, INDIA.

CORRESPONDENCE

Jagdish Manwar

Poona College of Pharmacy, Bharati Vidyapeeth University, Pune 411 038, INDIA.

jagdishmanwar@rediffmail.com

Received: 27-09-2013; Revised: 22-11-2013; Accepted: 16-12-2013.

Volume 5, Issue 4 · pp. 191–194 · PUBLISHED · DOI: 10.1016/j.jyp.2013.11.007

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ABSTRACT

Objective:To isolate and characterize the alcohol-producing yeasts from Woodfordia fruticosa flowers, which are used for the induction and maintenance of fermentation in the making of Ayurvedic formulations. Materials and methods:Initially twenty four yeasts strains were isolated on MGYP agar plate. Among them, four strains were selected for further studies on the basis of their alcohol generation capacity using jaggery media (50% w/v). Physiological, biochemical and genetic characterization (18S rRNA sequencing) of selected strains were carried out. Results: Physiological, biochemical and genetic characterization (18S rRNA sequencing) confirmed the strains as Saccharomycopsis fibuligera Jm.8, S. fibuligera Jm.10, S. fibuligera Jm.16 and Saccharomyces cerevisiae Jm.20. Under the controlled conditions, S. cerevisiae Jm.20 produced 69.57 g/l of alcohol, whereas remaining strains produced the alcohol in the range of 6.04e7.32 g/l. Conclusion: Among selected strains, strains S. fibuligera are a newer in the flowers. Kinetic study of alcohol generation revealed the strain S. cerevisiae Jm.20 can be efficiently used in making of fermented Ayurvedic formulations instead of use W. fruticosa flowers.

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    Manwar, J., Mahadik, K., Paradkar, A., Sathiyanarayanan, L., Vohra, M., & Patil, S. (). Isolation, biochemical and genetic characterizations of alcohol-producing yeasts from the flowers of Woodfordia fruticosa. Journal of Young Pharmacists, 5(4), 191–194. https://doi.org/10.1016/j.jyp.2013.11.007