Capillary Electrophoresis Method Development for Simultaneous Determination of Atorvastatin and Ezetimibe from Solid Dosage Form
Mahesh Attimarad1★★ Corresponding author
- 1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, KSA.
CORRESPONDENCE
Mahesh Attimarad
Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, KSA.
Received: 22-06-2016; Revised: 19-07-2016; Accepted: 07-08-2016.
Volume 9, Issue 1 · pp. 120–123 · PUBLISHED · DOI: 10.5530/jyp.2017.9.22
ABSTRACT
Purpose: A simple, rapid, validated capillary zone electrophoresis (CZE) was established for the simultaneous estimation of atorvastatin and ezetimibe from solid dosage form. Method: The separation of both the drugs was accomplished on fused silica capillary (48cm × 50µmi.d.) using background electrolyte consisting of 2mM borate buffer (pH 9.3) and methanol (80:20 v/v) as BGE. All the capillary conditions were optimized and newly developed method was validated as per the ICH guidelines. Results: Good linear calibration was obtained for both the analytes ranging from 5 to 200µg/ml and 2 to 20 µg/ml for atorvastatin and ezetimibe respectively. The limit of detection and quantification were less than 2 µg/ml for both the analytes. Repeatability and intra-day precision were well within the acceptable range. The accuracy was expressed as percent recovery, which is found to be between 98.20% to 100.41%.Conclusion:The newly developed simple and validated CZE method was used for simultaneous determina tion of atorvastatin and ezetimibe from solid dosage form.
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Attimarad, M. (). Capillary Electrophoresis Method Development for Simultaneous Determination of Atorvastatin and Ezetimibe from Solid Dosage Form. Journal of Young Pharmacists, 9(1), 120–123. https://doi.org/10.5530/jyp.2017.9.22