Cissus quadrangularis Linn. Stem Ethanolic Extract Liberates Reactive Oxygen Species and Induces Mitochondria Mediated Apoptosis in KB Cells
Saba Sheikh1, Sahabjada Siddiqui2, Anupam Dhasmana1, Safia Safia1, Ejazul Haque1, Mohammed Kamil1, Mohtashim Lohani1, Mohammad Arshad2, Snober Shabnam Mir1★★ Corresponding author
- 1Department of Bioengineering and Biosciences, Integral University.
- 2Department of Zoology, Lucknow University, Lucknow, Uttar Pradesh, India ORIGINAL ARTICLE.
CORRESPONDENCE
Snober Shabnam Mir
Department of Bioengineering and Biosciences, Integral University.
Volume 11, Issue 44S2 · pp. 365–374 · PUBLISHED 2015 · DOI: 10.4103/0973-1296.168972
ABSTRACT
Background: Cissus quadrangularis Linn. (CQ) commonly known as Hadjod (Family: Vitaceae) is usually distributed in India and Sri Lanka and contains several bioactive compounds responsible for various metabolic and physiologic effects. Objective: In this study, the biological effects of CQ ethanolic extract were evaluated by in vitro and supported by in silico analysis on KB oral epidermoid cancer cell line. Materials and Methods: Anti‑cancer potential of ethanolic extract of CQ stem against KB oral epidermoid cancer cells was evaluated in terms of morphological analysis, nuclei staining, liberation of reactive oxygen species (ROS), cell cycle arrest, mitochondrial membrane potential (MMP) and p53 and Bcl‑2 protein expression which reveal the induction of apoptosis along with supporting in silico analysis. Results: Ethanolic extract of CQ stem contains various bioactive compounds responsible for cancer cell morphological alterations, liberation of ROS, G1 phase cell cycle arrest and decreased MMP along with up‑regulation of p53 and down‑regulation of Bcl‑2. By employing in silico approach, we have also postulated that the CQ extract active constituents sequester Bcl‑2 with higher affinity as compared to p53, which may be the reason for induction of growth arrest and apoptosis in KB cells. Conclusion: Our data indicate that the CQ extract has a remarkable apoptotic effect that suggests that it could be a viable treatment option for specific types of cancers.
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Sheikh, S., Siddiqui, S., Dhasmana, A., Safia, S., Haque, E., Kamil, M., Lohani, M., Arshad, M., & Mir, S. S. (2015). Cissus quadrangularis Linn. Stem Ethanolic Extract Liberates Reactive Oxygen Species and Induces Mitochondria Mediated Apoptosis in KB Cells. Pharmacognosy Magazine, 11(44S2), 365–374. https://doi.org/10.4103/0973-1296.168972