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Article

A New Sucrase Enzyme Inhibitor from Azadirachta indica

Mohamed I. S. Abdelhady2,3★, Usama Shaheen2,4, Ammar Bader2, Mahmoud A. Youns5,6★ Corresponding author

  1. 1Department of Pharmacognosy, Faculty of Pharmacy, Umm Al‑Qura University, Makkah 21955, Departments of, Saudi Arabia.
  2. 2Pharmacognosy and.
  3. 3Department of Pharmacognosy, Faculty of Pharmacy, Al‑Azhar University, Cairo, Egypt.
  4. 4Biochemistry, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt.
  5. 5Division of Functional Genome Analysis, German Cancer Research Center, Heidelberg, Germany.

CORRESPONDENCE

Mohamed I. S. Abdelhady

Pharmacognosy and.

mohibrahem@yahoo.com

Received: 04-04-2015; Revised: 14-07-2015.

Volume 12, Issue 46S · pp. S293–S296 · PUBLISHED 7 July 2016 · DOI: 10.4103/0973-1296.185705

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ABSTRACT

Background: Sucrase enzyme inhibitor considered as an oral anti-diabetic therapy that delays the absorption of eaten carbohydrates, reducing the postprandial glucose and insulin peaks to reach normoglycemia. Materials and Methods: Chromatographic fractionation of the hydroalcoholic extract of leaves of Azadirachta indica growing in KSA, followed by in-vitro assay of sucrase enzyme inhibition activity. Results: This investigation led to the isolation of a new remarkable sucrase enzyme inhibitor; 4`-methyl Quercetin-7-O-β -D-glucuronopyranoside (1) alongside with four known compounds; 2,3-hexahydroxydiphenoyl-(α/β)-D-4C1-glucopyranose (2), Avicularin (3), Castalagin (4) and Quercetin-3-O-glucoside (5). The structure of the new compound (1) was elucidated on the basis of its spectral data, including ESI-MS, UV, 1H NMR, 13C NMR, 1H-1H COSY, HSQC, NOESY and HMBC. Conclusion: Under the assay conditions, hydroalcoholic extract of A. indica and compounds 1-5 exhibited significant sucrase enzyme inhibitory activity.

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    Abdelhady, M. I. S., Shaheen, U., Bader, A., & Youns, M. A. (2016). A New Sucrase Enzyme Inhibitor from Azadirachta indica. Pharmacognosy Magazine, 12(46S), S293–S296. https://doi.org/10.4103/0973-1296.185705