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Storing of Extracts in Polypropylene Microcentrifuge Tubes Yields Contaminant Peak During Ultra-flow Liquid Chromatographic Analysis

Parthraj R. Kshirsagar2, Harsha Hegde2,3, Sandeep R. Pai2★★ Corresponding author

  1. 1Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, India.
  2. 2Traditional and Herbal Medicine Division, Regional Medical Research Centre, Indian Council of Medical Research, Belagavi, Karnataka, India.

CORRESPONDENCE

Sandeep R. Pai

Traditional and Herbal Medicine Division, Regional Medical Research Centre, Indian Council of Medical Research, Belagavi, Karnataka, India.

drpaisr@gmail.com

Received: 12-01-2016; Revised: 10-02-2016.

Volume 12, Issue 46S · pp. S303–S306 · PUBLISHED 7 July 2016 · DOI: 10.4103/0973-1296.185719

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ABSTRACT

Aim: This study was designed to understand the effect of storage in polypropylene microcentrifuge tubes and glass vials during ultra- flow liquid chromatographic (UFLC) analysis. Materials and Methods: One ml of methanol was placed in polypropylene microcentrifuge tubes (PP material, Autoclavable) and glass vials (Borosilicate) separately for 1, 2, 4, 8, 10, 20, 40, and 80 days intervals stored at −4°C. Results: Contaminant peak was detected in methanol stored in polypropylene microcentrifuge tubes using UFLC analysis. The contaminant peak detected was prominent, sharp detectable at 9.176 ± 0.138 min on a Waters 250–4.6 mm, 4 μ, Nova-Pak C18 column with mobile phase consisting of methanol:water (70:30). Conclusion: It was evident from the study that long-term storage of biological samples prepared using methanol in polypropylene microcentrifuge tubes produce contaminant peak. Further, this may mislead in future reporting an unnatural compound by researchers.

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    Kshirsagar, P. R., Hegde, H., & Pai, S. R. (2016). Storing of Extracts in Polypropylene Microcentrifuge Tubes Yields Contaminant Peak During Ultra-flow Liquid Chromatographic Analysis. Pharmacognosy Magazine, 12(46S), S303–S306. https://doi.org/10.4103/0973-1296.185719