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Exploratory Studies of (-)-Epicatechin, a Bioactive Compound of Phyllanthus niruri, on the Antioxidant Enzymes and Oxidative Stress Markers in D-galactosamine-induced Hepatitis in Rats: A Study with Reference to Clinical Prospective

Bhasha Shanmugam1,2, Kondeti Ramudu Shanmugam2★, Sahukari Ravi1, Ganjikunta Venkata Subbaiah1, Chilakala Ramakrishana3, Korivi Mallikarjuna4, Kesireddy Sathyavelu Reddy1★ Corresponding author

  1. 1Division of Molecular Biology and Ethanopharmacology, Department of Zoology, Sri Venkateswara University, Tirupati.
  2. 2Department of Zoology, T.R.R. Government Degree College, Kandukur, Andhra Pradesh, India.
  3. 3Mineral Resources Research Division, Korea Institute of Geoscience, Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
  4. 4Institute of Nutrition (16F), China Medical University, Hsueh-Shih Road, North District, Taichung, Taiwan.

CORRESPONDENCE

Kondeti Ramudu Shanmugam

Department of Zoology, T.R.R. Government Degree College, Kandukur, Andhra Pradesh, India.

drshanmugam2016@gmail.com

Received: 16-07-2016; Revised: 26-08-2016; Accepted: 26-08-2016.

Volume 13, Issue 49S · pp. 56–62 · PUBLISHED 7 April 2017 · DOI: 10.4103/0973-1296.203973

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ABSTRACT

Background: Hepatitis is a health problem affecting millions of people worldwide and it is the major risk factor for liver cirrhosis. In India, many plants are used to treat hepatitis. But little is known about the effects of (-)-epicatechin a bioactive compound of Phyllanthus niruri (PN) in hepatitis rats. Objective: The present study was designed to explore the antioxidant property of (-)-epicatechin isolated from PN in D-Galactosamine (D-GalN) induced hepatitis rats. Materials and Methods: The rats are divided into five groups as per the experimental design. (-)-Epicatchin pretreatment was given to the hepatitis rats for 21 days and biochemical analysis was carried out. The hepatic antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), glutathione S-transferase (GST), reduced glutathione (GSH), and malondialdehyde (MDA) and serum markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), albumin, and bilirubin are estimated. Results: All the antioxidant enzymes activities and albumin levels are depleted in hepatitic rats. Whereas GST, ALP, AST, ALT activities and MDA, and bilirubin levels are elevated in hepatitis rats, (-)-epicatechin pretreatment increased all the antioxidant enzymes and decreased the GST, ALP, AST, ALT, and MDA levels in hepatitis rats. However, histopatholoigic studies also proves that (-)-epicatechin pretreatment decreased the tissue damage in hepatitis condition. This is the first report on the antioxidant enzymes and hepatoprotective effect of (-)-epicatechin in hepatitis rats. Conclusion: From this study, we conclude that (-)-epicatechin treatment decreased the oxidative damage in hepatitis rats.

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    Shanmugam, B., Shanmugam, K. R., Ravi, S., Subbaiah, G. V., Ramakrishana, C., Mallikarjuna, K., & Reddy, K. S. (2017). Exploratory Studies of (-)-Epicatechin, a Bioactive Compound of Phyllanthus niruri, on the Antioxidant Enzymes and Oxidative Stress Markers in D-galactosamine-induced Hepatitis in Rats: A Study with Reference to Clinical Prospective. Pharmacognosy Magazine, 13(49S), 56–62. https://doi.org/10.4103/0973-1296.203973