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Chemical Composition of Moringa oleifera Ethyl Acetate Fraction and Its Biological Activity in Diabetic Human Dermal Fibroblasts

Sivapragasam Gothai2, Katyakyini Muniandy2, Mazni Abu Zarin2, Tan Woan Sean2, S. Suresh Kumar3, Murugan A. Munusamy4, Sharida Fakurazi2,5, Palanisamy Arulselvan2★★ Corresponding author

  1. 1Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia.
  2. 2Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Institute of Bioscience, Universiti Putra Malaysia.
  3. 3Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
  4. 4Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Institute of Bioscience, Universiti Putra Malaysia, Malaysia.

CORRESPONDENCE

Palanisamy Arulselvan

Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Institute of Bioscience, Universiti Putra Malaysia.

arulbio@gmail.com

Received: 12-08-2016; Revised: 08-11-2016.

Volume 13, Issue 51S · pp. S462–S469 · PUBLISHED 11 October 2017 · DOI: 10.4103/pm.pm_368_16

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ABSTRACT

Background: Moringa oleifera (MO), commonly known as the drumstick tree, is used in folklore medicine for the treatment of skin disease. Objective: The objective of this study is to evaluate the ethyl acetate (EtOAc) fraction of MO leaves for in vitro antibacterial, antioxidant, and wound healing activities and conduct gas chromatography‑mass spectrometry (GC‑MS) analysis. Materials and Methods: Antibacterial activity was evaluated against six Gram‑positive bacteria and 10 Gram‑negative bacteria by disc diffusion method. Free radical scavenging activity was assessed by 1, 1‑diphenyl‑2‑picryl hydrazyl (DPPH) radical hydrogen peroxide scavenging and total phenolic content (TPC). Wound healing efficiency was studied using cell viability, proliferation, and scratch assays in diabetic human dermal fibroblast (HDF‑D) cells. Results: The EtOAc fraction showed moderate activity against all bacterial strains tested, and the maximum inhibition zone was observed against Streptococcus pyogenes (30 mm in diameter). The fraction showed higher sensitivity to Gram‑positive strains than Gram‑negative strains. In the quantitative analysis of antioxidant content, the EtOAc fraction was found to have a TPC of 65.81 ± 0.01. The DPPH scavenging activity and the hydrogen peroxide assay were correlated with the TPC value, with IC50 values of 18.21 ± 0.06 and 59.22 ± 0.04, respectively. The wound healing experiment revealed a significant enhancement of cell proliferation and migration of HDF‑D cells. GC‑MS analysis confirmed the presence of 17 bioactive constituents that may be the principal factors in the significant antibacterial, antioxidant, and wound healing activity. Conclusion: The EtOAc fraction of MO leaves possesses remarkable wound healing properties, which can be attributed to the antibacterial and antioxidant activities of the fraction.

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    Gothai, S., Muniandy, K., Zarin, M. A., Sean, T. W., Kumar, S. S., Munusamy, M. A., Fakurazi, S., & Arulselvan, P. (2017). Chemical Composition of Moringa oleifera Ethyl Acetate Fraction and Its Biological Activity in Diabetic Human Dermal Fibroblasts. Pharmacognosy Magazine, 13(51S), S462–S469. https://doi.org/10.4103/pm.pm_368_16