Evaluation of Hypolipidemic and Antioxidant Effects in Phenol-rich Fraction of Crataegus pinnatifida Fruit in Hyperlipidemia Rats and Identification of Chemical Composition by Ultra-performance Liquid Chromatography Coupled with Quadropole Time-of-flight Mass Spectrometry
Feng Shao1, Lifei Gu2, Huijuan Chen3, Ronghua Liu3, Huilian Huang3, Lanying Chen3, Ming Yang3★★ Corresponding author
- 1Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, China.
- 2Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, Jiangsu, China.
- 3Department of Pharmacy, The People’s Hospital of Bozhou, Anhui, China.
CORRESPONDENCE
Ming Yang
Department of Pharmacy, The People’s Hospital of Bozhou, Anhui, China.
Received: 12-09-2016; Revised: 03-11-2016; Accepted: 03-11-2016.
Volume 13, Issue 52 · pp. 725–731 · PUBLISHED 13 November 2017 · DOI: 10.4103/pm.pm_402_16
ABSTRACT
Background: Hawthorn (Crataegus pinnatifida) fruit has enjoyed a great popularity as a pleasant-tasting food associated with hypolipidemic and antioxidant effects. Objective: Our aim was to screen the effective fraction of hawthorn fruit in the treatment of hyperlipidemia rats. Materials and Methods: In this study, ethanol extract of hawthorn fruit (Fr.1) and four fractionated extracts (Fr.2, Fr.3, Fr.4, and Fr.5) were compared to total phenol content evaluated using Folin–Ciocalteu method, and hypolipidemic and antioxidant effects were assessed in hyperlipidemic rats. Results: Total phenol content of Fr.4 was higher than other fractions by at least 2 fold. Furthermore, this fraction possessed the strongest hypolipidemic and antioxidant effects in hyperlipidemic rats. On this basis, 15 phenolic compounds and four organic acids in Fr.4 were positively or tentatively identified using ultra-performance liquid chromatography coupled with quadropole time-of-flight mass spectrometry. In addition, 5-O-caffeoyl quinic acid butyl ester was first reported in hawthorn fruit. Conclusion: Phenol-rich fraction in hawthorn fruit exhibited satisfactory hypolipidemic and antioxidant effects, and this could be exploited for further promotion of functional foods.
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Shao, F., Gu, L., Chen, H., Liu, R., Huang, H., Chen, L., & Yang, M. (2017). Evaluation of Hypolipidemic and Antioxidant Effects in Phenol-rich Fraction of Crataegus pinnatifida Fruit in Hyperlipidemia Rats and Identification of Chemical Composition by Ultra-performance Liquid Chromatography Coupled with Quadropole Time-of-flight Mass Spectrometry. Pharmacognosy Magazine, 13(52), 725–731. https://doi.org/10.4103/pm.pm_402_16