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Ultra‑performance Liquid Chromatography/Quadrupole Time‑of‑flight Mass Spectrometry Analysis of In vitro Metabolites of Lignans from Fructus Forsythiae by Human Fecal Flora

Shihua Xing1, Chongsheng Peng1, Mengyue Wang1, Ying Peng1, Xiaobo Li1★★ Corresponding author

  1. 1School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.

CORRESPONDENCE

Xiaobo Li

School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.

xbli@sjtu.edu.cn

Received: 02-05-2017; Revised: 03-06-2017.

Volume 14, Issue 56 · pp. 364–370 · PUBLISHED 14 August 2018 · DOI: 10.4103/pm.pm_177_17

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ABSTRACT

Background: Phillyrin and (+)‑pinoresinol 4‑O‑‑D‑glucoside are the major active furofuran‑type lignans in Fructus Forsythiae. The metabolic routes and metabolites of these two lignans are not well understood yet. Objective: In this study, we attempted to identify the human‑intestine bacterial metabolites of lignans from Fructus Forsythiae. Materials and Methods: Two natural compounds, phillyrin and (+)‑pinoresinol 4‑O‑‑D‑glucoside were incubated with human fecal microflora in an anaerobic incubator for 72 h and the metabolites with highly sensitive ultra‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry (UPLC‑Q‑TOF/MS) were analyzed. Results: As a result, nine metabolites were determined using a Syncronis™ C18 column (particle size 1.7 mm) in a gradient elution system. These metabolites were then identified according to the mass fragmental mechanism, MS/MS fragment ions, and previous publications. The results of this study indicated that the major metabolites of furofuran‑type lignans are through the processes of hydrolysis, demethylation, reduction, dehydroxylation, and oxidation. Conclusions: Lignans can be metabolized by intestinal microbiota and the intestinal bacteria play a critical role in the metabolism of components administered orally.

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    Xing, S., Peng, C., Wang, M., Peng, Y., & Li, X. (2018). Ultra‑performance Liquid Chromatography/Quadrupole Time‑of‑flight Mass Spectrometry Analysis of In vitro Metabolites of Lignans from Fructus Forsythiae by Human Fecal Flora. Pharmacognosy Magazine, 14(56), 364–370. https://doi.org/10.4103/pm.pm_177_17