Propionic Acid Abrogates the Deleterious Effects of Cerebral Ischemic Reperfusion Injury through Nuclear Factor‑κb Signaling in Mice
Tiansong Yang1★, Yulin Wang2★, Chaoran Li2★, Yan Yang2★, Delong Wang2★, Jing Guo2, Tianyu Shi2, Yue Wang2, Yuanyuan Qu2, Qingshuang Wei2, Chuwen Feng2, Zhongren Sun2★★ Corresponding author
- 1Department of Neurology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, China.
- 2Heilongjiang University of Chinese Medicine, Xiangfang District, Harbin, Heilongjiang, China.
CORRESPONDENCE
Tiansong Yang
Department of Neurology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, China.
Received: 19-07-2019; Revised: 14-08-2019.
Volume 16, Issue 67 · pp. 177–182 · PUBLISHED 11 February 2020 · DOI: 10.4103/pm.pm_306_19
ABSTRACT
Background: Cerebral ischemia is caused due to insufficient blood flow to brain cells. This study evaluates the therapeutic effects of propionic acid (PA) on the abnormalities induced during the cerebral ischemic‑reperfusion (CIR) injury in mice. Materials and Methods: CIR was induced by complete occlusion of the middle artery of the cerebrum for 15 min and reperfusion for 24 h in the experimental C57BL/6 mice. The analysis of mRNA levels of neuronal nitric oxide synthase (nNOS) was performed by reverse transcriptase polymerase chain reaction (RT‑PCR). Inflammatory cytokines such as interleukin‑6 (IL‑6), IL‑1 β, and tumor necrosis factor‑alpha were quantitated by enzyme‑linked immuno sorbent assay. Apoptosis‑related proteins and nuclear factor‑κB (NF‑κB) were analyzed using Western blot technique. Results: PA demonstrated strong therapeutic effects on controlling the expression of nNOS that was analyzed using RT‑PCR. Elevated levels of inflammatory cytokines, caspase‑3, caspase‑9, and phosphorylated NF‑κB during cerebral ischemia were significantly controlled during PA treatment. Conclusion: Our findings demonstrated that PA through oral gavage exhibited healthier effects on the damage caused due to CIR injury.
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Yang, T., Wang, Y., Li, C., Yang, Y., Wang, D., Guo, J., Shi, T., Wang, Y., Qu, Y., Wei, Q., Feng, C., & Sun, Z. (2020). Propionic Acid Abrogates the Deleterious Effects of Cerebral Ischemic Reperfusion Injury through Nuclear Factor‑κb Signaling in Mice. Pharmacognosy Magazine, 16(67), 177–182. https://doi.org/10.4103/pm.pm_306_19