Vanillin an Active Constituent from Vanilla Bean Induces Apoptosis and Inhibits Proliferation in Human Colorectal Adenocarcinoma Cell Line
Yandong Xie1, Jingyu Zhang1, Cheng Wang1, Qinglin Fan1, Yiling Zhang2★★ Corresponding author
- 1Departments of Gastroenterology, Hospital, Fourth Military Medical University, Baqiao, Xi’an, Shaanxi Province, China.
- 2Oncology, Tangdu Hospital, Fourth Military Medical University, Baqiao, Xi’an, Shaanxi Province, China.
CORRESPONDENCE
Yiling Zhang
Oncology, Tangdu Hospital, Fourth Military Medical University, Baqiao, Xi’an, Shaanxi Province, China.
Received: 01-06-2019; Revised: 14-08-2019.
Volume 16, Issue 67 · pp. 197–200 · PUBLISHED 11 February 2020 · DOI: 10.4103/pm.pm_235_19
ABSTRACT
Aim: The aim of the study was to investigate the antiproliferative and proapoptotic effects of vanillin (VL) an active constituent from Vanilla bean in colon cancer (human colorectal adenocarcinoma cell line [HT-29]) cells. Methodology: We demonstrated the cell growth and apoptosis in HT-29 cells. The apoptotic measures were analyzed by analyzing mitochondrial membrane potential (Δψm) level; apoptotic bodies by acridine orange/ethidium bromide and Hoechst staining. Results: Our results indicated that VL induces apoptosis as evidenced by cell viability loss, resulting in the altered Δψm in HT-29 cells. Further, the prooxidant role of VL inhibit B-cell lymphoma 2 (Bcl2) expression with the simultaneous upregulation of BCL-2 associated X, Cytochrome c, Capspase-9 and 3 protein expressions in HT-29 cells. Conclusion: Thus, in this regard, we have concluded that VL induces apoptosis in HT-29 cells through inducing oxidative damage and modulates apoptotic marker expressions. VL may therefore be used as a therapeutic agent for colon cancer treatment.
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Xie, Y., Zhang, J., Wang, C., Fan, Q., & Zhang, Y. (2020). Vanillin an Active Constituent from Vanilla Bean Induces Apoptosis and Inhibits Proliferation in Human Colorectal Adenocarcinoma Cell Line. Pharmacognosy Magazine, 16(67), 197–200. https://doi.org/10.4103/pm.pm_235_19