Anwulignan from the Fruits of Schisandra chinensis and its Cytotoxicity against Human Cancer Cell Lines
Yeong‑Geun Lee1, Rong Bo Wang1, Hyoung‑Geun Kim1, Dae‑Young Lee2, Yeon‑Ju Kim1, Nam‑In Baek1★★ Corresponding author
- 1Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin‑Si.
- 2Herbal Science, RDA, Eumseong‑gun, Korea.
CORRESPONDENCE
Nam‑In Baek
Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin‑Si.
Received: 18-05-2020; Revised: 15-07-2020; Accepted: 21-09-2020.
Volume 16, Issue 72 · pp. 695–699 · PUBLISHED 16 February 2021 · DOI: 10.4103/pm.pm_208_20
ABSTRACT
Objectives: We reviewed for the effects of cytotoxic lignans against various human cancer cells. Materials and Methods: Solvent extraction of fruits in aqueous methanol and fractionation of the extract into H2O, n‑BuOH, and EtOAc fractions (Fr) were carried out. Column chromatography (CC) of the non‑polar EtOAc Fr, which indicated cytotoxicity to cancer cells, was performed to isolate the cytotoxic lignin. They were identified on the basis of the intensive spectroscopic interpretation of IR, 1D‑, 2D‑NMR, and mass spectrometry data. The cytotoxicity was evaluated by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay and cell staining using Hoechst 32258 and propidium iodide. Results: Anwulignan was isolated and identified as a cytotoxic principal component. The IC50 value was calculated to be 22.01 ± 1.87, 156.04 ± 6.71, and 32.68 ± 2.21 μM for human stomach adenocarcinoma, human colon cancer (HT29), and human cervical cancer cells, respectively. Conclusion: Solvent extraction, systematic fractionation, and repeated CC for S. chinensis fruits led to the isolation of a lignin identified to be anwulignan based on various spectroscopic analyses. Anwulignan demonstrated very high cytotoxicity to most human cancer cell lines, and cell death was induced by apoptosis.
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Lee, Y., Wang, R. B., Kim, H., Lee, D., Kim, Y., & Baek, N. (2021). Anwulignan from the Fruits of Schisandra chinensis and its Cytotoxicity against Human Cancer Cell Lines. Pharmacognosy Magazine, 16(72), 695–699. https://doi.org/10.4103/pm.pm_208_20