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Pharmacognosy MagazineNOW IN
Article

Salidroside Induces Apoptosis and Autophagy in Gastric Cancer Cells via Regulation of Mitogen‑Activated Protein Kinases Signaling Pathway

Canhong Hu2,3★, Shao Xiang4★, Lingchang Li2,3, Feng Xia Qin2,3, Guoli Wei2,3, Xiaofei Huang2,3, Rong Ding2,3, Jiege Huo2,3★, Zhijun Fang2,3★ Corresponding author

  1. 1Department of Oncology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, China.
  2. 2Department of Oncology, Jiangsu Province Academy of Traditional Chinese Medicine, Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, China.
  3. 3Graduate School, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210046, China.

CORRESPONDENCE

Canhong Hu

Department of Oncology, Jiangsu Province Academy of Traditional Chinese Medicine, Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, China.

huojiege@sina.com

Received: 30-03-2020; Revised: 22-04-2020; Accepted: 21-01-2021.

Volume 17, Issue 73 · pp. 179–185 · PUBLISHED 15 April 2021 · DOI: 10.4103/pm.pm_119_20

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ABSTRACT

Background: Salidroside, an active ingredient of Rhodiola rosea, exhibits antiproliferative effect in gastric cancer (GC) cells. However, the involvement of salidroside in apoptosis and autophagy of GC cells has not been elucidated. Materials and Methods: Cell viability of BGC‑823 cells was assessed by CCK‑8 assay, and apoptosis was analyzed with terminal deoxyribonucleotidyl transferase‑mediated dUTP nick‑end labeling assay and flow cytometry. The apoptosis‑ and autophagy‑associated proteins and mitogen‑activated protein kinases (MAPKs) were determined by Western blot analysis. Autophagy was evaluated by green fluorescent protein‑fused LC3 punctate formation. Results: Salidroside inhibited proliferation and promoted apoptosis and autophagy of BGC‑823 cells in a dose‑dependent manner. In addition, salidroside enhanced the phosphorylation of p38 MAPK, ERK1/2, and JNK. Furthermore, inhibition of MAPKs significantly abolished the effects of salidroside in BGC‑823 cells. Conclusion: Salidroside induced apoptosis and autophagy of BGC‑823 cells via activation of MAPK signaling pathway.

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    Hu, C., Xiang, S., Li, L., Qin, F. X., Wei, G., Huang, X., Ding, R., Huo, J., & Fang, Z. (2021). Salidroside Induces Apoptosis and Autophagy in Gastric Cancer Cells via Regulation of Mitogen‑Activated Protein Kinases Signaling Pathway. Pharmacognosy Magazine, 17(73), 179–185. https://doi.org/10.4103/pm.pm_119_20