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Pharmacognosy MagazineNOW IN
Article

Genetic Clonal Fidelity Assessment of Rhizome‑Derived Micropropagated Acorus calamus L. – A Medicinally Important Plant by Random Amplified Polymorphic DNA and Inter‑Simple Sequence Repeat Markers

Leimapokpam Tikendra1, Oinam Sushma1, Thoungamba Amom1, Nandeibam Apana Devi1, Sonia Paonam1, Nongthombam Bidyananda1, Angamba Meetei Potshangbam1, Abhijit Dey2, Rajkumari Sanayaima Devi3, Potshangbam Nongdam1★★ Corresponding author

  1. 1Department of Biotechnology, Manipur University, Canchipur, Imphal- 795003, Manipur, India.
  2. 2Department of Life Sciences, Presidency University, Kolkata, West Bengal, India.
  3. 3Department of Botany, Deen Dayal Upadhyaya College, University of Delhi, New Delhi, India.

CORRESPONDENCE

Potshangbam Nongdam

Department of Biotechnology, Manipur University, Canchipur, Imphal- 795003, Manipur, India.

nongpuren@gmail.com

Received: 01-09-2021; Revised: 10-11-2021; Accepted: 04-01-2022.

Volume 18, Issue 77 · pp. 207–215 · PUBLISHED 28 March 2022 · DOI: 10.4103/pm.pm_408_21

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ABSTRACT

Objectives: Development of fast and reliable in vitro regeneration protocol for A. calamus and clonal fidelity assessment of the regenerants using molecular markers. Materials and Methods: Plants were regenerated on Murashige and Skoog medium with different concentrations of growth regulators in two phases – shooting and rooting. Random amplified polymorphic DNA (RAPD) and inter‑simple sequence repeat (ISSR) markers were employed to evaluate the genetic stability of in vitro clones. Results: 6 Benzylaminopurine (BAP) at 1.6 and 2.4 mgL−1 was effective for shoot induction, while root induction was superior in indole‑3‑butyric acid‑incorporated medium at 2.5 mgL− . Thirteen RAPD and 16 ISSR primers produced 59 and 96 clear, unambiguous, and reproducible bands, respectively. Both the markers revealed a high monomorphism of 96.79% and 95.63% among the regenerants. Nei’s genetic distance analysis disclosed a close genetic association (0.000–0.068) among the genotypes. Conclusion: ISSR was better than RAPD markers in clonal fidelity assessment of the regenerants. The in vitro protocol developed is reliable and suitable for the rapid propagation of true‑to‑type A. calamus plants.

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    Tikendra, L., Sushma, O., Amom, T., Devi, N. A., Paonam, S., Bidyananda, N., Potshangbam, A. M., Dey, A., Devi, R. S., & Nongdam, P. (2022). Genetic Clonal Fidelity Assessment of Rhizome‑Derived Micropropagated Acorus calamus L. – A Medicinally Important Plant by Random Amplified Polymorphic DNA and Inter‑Simple Sequence Repeat Markers. Pharmacognosy Magazine, 18(77), 207–215. https://doi.org/10.4103/pm.pm_408_21