Degalactotigonin Inhibits Invasion and Induce Apoptosis by Targeting TGF‑β Signalling in Oral Cancer Cells
Yang Li2, Chengnan Gu3★★ Corresponding author
- 1Department of Orthodontics, Daqing Oilfield General Hospital, Daqing, Heilongjiang, 163001, China.
- 2Department of Stomatology, Nantong Haimen People’s Hospital, Nantong, Jiangsu, 226100, China.
Received: 07-01-2022; Revised: 02-04-2022; Accepted: 01-08-2022.
Volume 18, Issue 80 · pp. 1202–1210 · PUBLISHED 23 November 2022 · DOI: 10.4103/pm.pm_7_22
ABSTRACT
Objectives: The study aimed to investigate the anti‑invasion and apoptotic induction effect of DGT in oral squamous cell carcinoma (OSCC) cells through inhibiting non‑canonical TGF‑β signalling. Materials and Methods: Human oral cancer KB (KERATIN-forming tumor cell line HeLa) cells were chosen to study the anti‑cancer activity of DGT in vitro experiments. The cytotoxic effect of DGT was evaluated by MTT (3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assay. Cell growth, DNA damage, invasion inhibition and apoptosis activation were evaluated by mitochondrial membrane potential (ΔΨM), comet assay, reactive oxygen species (ROS) and AO/EtBr staining. The DGT effect on protein expression levels related to invasion, apoptotic markers and its activation signalling pathways on KB cells were examined by western blotting. Results: We found that DGT antioxidant properties reduced cell viability, generates ROS, enhanced DNA damage, and MMP dissipation. Further, TGF‑β inhibitions resulted in a reduction of extracellular signal‑regulated kinase (ERK), NF‑κB and activation of JNK, p38 which increase ROS in a cancer cell that downregulates cyclin‑D1, PCNA, MMP‑2, MMP‑9, Bcl‑2 and increases Bax, Caspase‑9, Caspase‑3 protein expressions that simultaneously subdues the cancer developments. Conclusion: These findings suggest that DGT inhibiting TGF‑β mediated ERK, NF‑κB and activation of JNK, p38 caused tumour cell death via ROS stimulation and apoptosis.
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Li, Y., & Gu, C. (2022). Degalactotigonin Inhibits Invasion and Induce Apoptosis by Targeting TGF‑β Signalling in Oral Cancer Cells. Pharmacognosy Magazine, 18(80), 1202–1210. https://doi.org/10.4103/pm.pm_7_22