jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Pharmacognosy MagazineNOW IN
Article

Study on the Mechanism of Anti-atopic Dermatitis by Herba Siegesbeckiae Based on System Pharmacology

Xiaowei Chen1,2, YingYue Wang1,2, Xin Zhang2, Chang Liu2, Chunxue You1, Yubin Xu2★★ Corresponding author

  1. 1Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, CHINA.
  2. 2Department of Pharmacy, Taizhou Central Hospital, Taizhou University Hospital, Taizhou, Zhejiang, China Xiaowei Chen and Yingyue Wang contributed equally to this work. Corresponding author: Yubin Xu, Department of Pharmacy, Taizhou Central Hospital, Taizhou University Hospital, Taizhou, Zhejiang 318000, China. E-mail: xuyubin1988@126.com.

CORRESPONDENCE

Yubin Xu

Department of Pharmacy, Taizhou Central Hospital, Taizhou University Hospital, Taizhou, Zhejiang, China.

xuyubin1988@126.com

Received: 06-11-2023; Accepted: 05-03-2024.

Volume 20, Issue 3 · pp. 1013–1022 · PUBLISHED 2024 · DOI: 10.1177/09731296241242172

View on PM original site ↗

ABSTRACT

Background: Atopic dermatitis (AD) is a common but complex chronic inflammatory skin condition characterised by intense pruritus, severely impacting patients’ quality of life. At present, there is no specific drug for AD. Herba Siegesbeckiae (HS) had a therapeutic effect on AD, but its mechanism has not been completely elucidated. Purpose: We aimed to understand the mechanism of HS in the treatment of AD in this study. Materials and Methods: To investigate the mechanism of HS for AD, including active components, key targets and pathway analyses, systemic pharmacology was used. Molecular docking was conducted to validate the interactions between the key components and their targets. Afterwards, an AD-like animal model was established, skin pathology was observed and immunohistochemical staining was used to confirm the key targets. Results: Six vital active components (daucosterol, d-mannitol, hythiemoside B, 15,16-di-O-acetyldarutoside, 19-acetoxy-15- hydroperoxy-12-oxo-13,14E-dehydro-10,11,14,15-tetrahydr-ogeranylnerol and 17-O-acetyldarutoside) highly correlated with potential targets and the key targets (TNF-α, VEGFA, TLR4, STAT3, TLR2, STAT1, MMP9, IL-6, TRPV1 and PPARG) highly correlated with disease processes were identified, pathway enrichment analysis revealed that the toll-like receptor, PI3K-Akt and HIF-1 signalling pathways were significantly enriched with key targets, suggesting their involvement in the anti-Alzheimer’s disease mechanism of HS. As a result of animal experiments, AD-like mice from the model group showed epidermal thickening and inflammatory cell infiltration; TNF-α, VEGFA, TLR4, STAT3, TLR2, STAT1, MMP9, IL-6, TRPV1 levels are significantly upregulated and PPARG levels were significantly down-regulated; those of which could be reversed by 3.0 g/kg. Conclusion: HS might exert its anti-AD effects by up-regulating PPARG and down-regulating TNF-α, VEGFA, TLR4, STAT3, TLR2, STAT1, MMP9, IL-6 and TRPV1. This study laid a foundation for understanding HS’s bioactive components and mechanism against AD.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Chen, X., Wang, Y., Zhang, X., Liu, C., You, C., & Xu, Y. (2024). Study on the Mechanism of Anti-atopic Dermatitis by Herba Siegesbeckiae Based on System Pharmacology. Pharmacognosy Magazine, 20(3), 1013–1022. https://doi.org/10.1177/09731296241242172