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Orthogonal test design for optimization of the extraction of polysaccharide from Paeonia sinjiangensis K.Y. Pan

Shuge Tian2,3★, Xiaoying Zhou4, Haiyan Gong5, Xiuming Ma5, Fan Zhang2,3★ Corresponding author

  1. 1Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, China.
  2. 2College of TCM, China.
  3. 3College of Pharmacy, China.
  4. 4College of Basic Medical, XinJiang Medical University, Urumqi - 830 011, XinJiang, China.

CORRESPONDENCE

Shuge Tian

College of TCM, China.

tianshuge@hotmail.com

Received: 13-07-2010; Revised: 18-09-2010.

Volume 7, Issue 25 · pp. · PUBLISHED 20 January 2011 · DOI: 10.4103/0973-1296.75874

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ABSTRACT

Background: Paeonia sinjiangensis K.Y. Pan is a perennial herb belonging to the ranunculaceae family and it is one of the most important crude drugs in traditional Chinese medicine. In this article, Paeonia sinjiangensis K.Y. Pan rich in polysaccharide is used as an experimental material. Materials and Method: Study the effects of proportion, temperature, times and time taken for the extraction yield of polysaccharide through a single-factor exploration. Then, through an orthogonal experiment (L9 (3)4), it was investigated to get the best extraction conditions. Results: The results showed that the ratio of solvent to raw material, number of extractions and duration of extraction were the main variables that influenced the yields of extracts. The separation procedure of precipitation with alcohol and the purification from the removing proteins were deeply analyzed. Meanwhile the contents of polysaccharide were determined by anthrone colorimetry. Conclusion: The highest yield was obtained when the ratio of solvent to raw material, number of extractions, and duration of extraction were 8:1, 2, and 1.5 h, respectively. The content of soluble polysaccharide is 51.57%.

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    Tian, S., Zhou, X., Gong, H., Ma, X., & Zhang, F. (2011). Orthogonal test design for optimization of the extraction of polysaccharide from Paeonia sinjiangensis K.Y. Pan. Pharmacognosy Magazine, 7(25). https://doi.org/10.4103/0973-1296.75874