Comparative HPTLC Analysis of Piperine Content in Roots of Piper longum L. Cultivated with Bio Formulations

ABSTRACT

Background: Phytochemicals from medicinal plants significantly impact healthcare. Piperine, a bioactive compound in Piper longum, exhibits diverse pharmacological effects, including analgesic, antipyretic, antioxidant, immune-stimulant, hepatoprotective, and bioavailability-enhancing properties. Traditionally, Piperineis extracted from both fruits (Pippali) and roots (Pippalimula) of Piper longum. Objectives:To quantify the Piperine content in Piper longum roots cultivated using bio formulations-Kunapa Jala and Vermiwash-on Piperine yield. Materials and Methods: Piper longumwas cultivated with Random Block Design with two treatment groups (Kunapa Jala and Vermiwash), each with 12 replications. Roots were harvested, cleaned, dried, and powdered. High-Performance Thin-Layer Chromatography (HPTLC) was used to detect and quantify of Piperine. Results: HPTLC analysis revealed the presence of Piperine in both groups. Quantitative comparison showed a higher Piperine concentration in the Kunapa Jala treated group than in the Vermiwash group. Conclusion: Kunapa Jala enhances Piperine content in Piper longum roots in comparison to Vermiwash. This supports the potential of Vrukshayurveda and traditional formulations in improving the phytochemical yield of Pippali.

KEYWORDS

• Piper longum
• Piperine
• HPTLC
• Kunapa Jala
• Vermiwash
• Vrukshayurveda

Showing references and in-text citations exactly as published.

• DOI10.5530/pres.20252328Copy

RELATED ARTICLES

Matched on section and article type within this issue of PR.

• Article · VOLUME 17, ISSUE 4Comparative LC-MS Profiling of two Linderniaceae SpeciesThachan Sreelakshmi, Meethaley Valappil Jeeshna, Abhijith Sarga, Sugunan Ashitha · pp. 1187–1196Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 4Investigating the Expression of Apoptosis and Metastasis Genes in Satureja khuzistanica along with Nisin and Doxorubicin in Colorectal CancerEsmaeil Kheiri, Zahra Hojjati Bonab, Hossein Soltanzadeh · pp. 1197–1204Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 4Nephroprotective and Cytotoxic Assessment of Moringa concanensis Leaf Extract in Gentamicin-Induced Renal Injury in RatsAbhishek Raghavendra, Hariprasad Madakasira Guggilla, Diya Lakshman Shetty, Deeksha Giridhar · pp. 1205–1213Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 4Evaluation of Anti-Depressant Activity of Garcinia cambogia Fruit Using Animal ModelsJ. Priyadharisini, N. Savitha, Lydia Jeeboi Paramel · pp. 1214–1217Original ArticleSame type: Article