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Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites

Gaurav Mahesh Doshi1,2★, Sandeep Prabhakar Zine1, Pratip Kashinath Chaskar1, Hemant Devidas Une3★ Corresponding author

  1. 1Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra
  2. 2Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan
  3. 3Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabaugh, Aurangabad, Maharashtra, India

CORRESPONDENCE

Gaurav Mahesh Doshi

*Address for correspondence: Asst. Prof. Gaurav M. Doshi, Department of Pharmacology, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra, India.

gaurav.pharmacology@gmail.com

Revised: 02-01-2014.

Volume 6, Issue 3 · pp. 234–239 · PUBLISHED July-September 2014 · DOI: 10.4103/0974-8490.132601

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ABSTRACT

Background Polyalthia longifolia Thwaites is an important traditional plant in India. Rutin, an active constituent has been reported to possess good amount of pharmacological as well as therapeutic potential. Objective The aim of the present study was to find out by analytical techniques how much percentage of rutin is present in the plant leaves’ ethanolic extract by analytical techniques. Materials and Methods Shade dried leaves of Polyalthia longifolia were subjected to cold ethanolic extraction followed by monitoring the isolated rutin high-pressure liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) after carrying out preliminary phytochemical screening. Results Extraction yield was found to be 13.94% w/w. Phytochemical screening of the extract showed the presence of flavonoids, steroids, diterpenoids, alkaloids, saponins, tannins and phenolic compounds and mucilage. From the Rf value, the ethanolic extract was found to be having constituent identical to rutin. By HPTLC and HPLC the amount of rutin was found to be 11.60% w/w and 4.03% w/v, respectively. Conclusion The active constituent isolated was found to be equal to rutin.

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    Doshi, G. M., Zine, S. P., Chaskar, P. K., & Une, H. D. (2014). Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites. Pharmacognosy Research, 6(3), 234–239. https://doi.org/10.4103/0974-8490.132601